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. 1983 Mar;153(3):1287–1293. doi: 10.1128/jb.153.3.1287-1293.1983

Labeling pattern of major penicillin-binding proteins of Escherichia coli during the division cycle.

F B Wientjes, T J Olijhoek, U Schwarz, N Nanninga
PMCID: PMC221775  PMID: 6337993

Abstract

Escherichia coli cells were synchronized by the elutriation technique. The pattern of penicillin-binding proteins (PBPs) in synchronously growing cells was determined with an iodinated derivative of ampicillin in intact cells as well as in isolated membranes. This was done under nonsaturating conditions as well as under conditions in which the PBPs were saturated with [125I]ampicillin. No evidence was found for fluctuations in the PBP pattern: the PBPs seem to be present in a constant ratio throughout the division cycle. The E. coli cells exert their control on shape maintenance and cell wall growth apparently not on the level of concentration of PBPs in the cell but rather on activation of existing components.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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