Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Oct;64(2):451–458. doi: 10.1073/pnas.64.2.451

INTERBASE VIBRATIONAL COUPLING IN G:C POLYNUCLEOTIDE HELICES

F B Howard 1,2, J Frazier 1,2, H Todd Miles 1,2
PMCID: PMC223363  PMID: 5261027

Abstract

The carbonyl stretching vibrations of G and C residues undergo frequency changes when a helical complex is formed from nonordered G and C components. We have prepared a series of two-stranded G:C helices having chemical and isotopic (18O) substitutions which lead to variation of the observed vibrational frequencies. Infrared spectra of these helices clearly demonstrate that vibrational coupling occurs between the guanine and cytosine residues in the helix. As a result of this coupling through the hydrogen bonds, the two carbonyl bands undergo frequency shifts of equal magnitude and opposite sign, with mixing of G and C character in the observed interacted vibrations.

Full text

PDF
452

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. GRUNBERG-MANAGO M., MICHELSON A. M. POLYNUCLEOTIDE ANALOGUES. II. STIMULATION OF AMINO ACID INCORPORATION BY POLYNUCLEOTIDE ANALOGUES. Biochim Biophys Acta. 1964 Mar 23;80:431–440. doi: 10.1016/0926-6550(64)90145-8. [DOI] [PubMed] [Google Scholar]
  2. HOWARD F. B., MILES H. T. 6-18-O-PURINE NUCLEOTIDES. SYNTHESIS AND INFRARED BAND ASSIGNMENT. J Biol Chem. 1965 Feb;240:801–805. [PubMed] [Google Scholar]
  3. Howard F. B., Frazier J., Miles H. T. Interaction of poly-5-bromocytidylic acid with polyinosinic acid. A study of helix stability and spectroscopic properties. J Biol Chem. 1969 Mar 10;244(5):1291–1302. [PubMed] [Google Scholar]
  4. Howard F. B., Frazier J., Singer M. F., Miles H. T. Helix formation between polyribonucleotides and purines, purine nucleosides and nucleotides. II. J Mol Biol. 1966 Apr;16(2):415–439. doi: 10.1016/s0022-2836(66)80183-3. [DOI] [PubMed] [Google Scholar]
  5. MILES H. T. Quantitative infrared spectra in D20 of some nucleosides, nucleotides, and polynucleotides; a new measure of polynucleotide interaction. Biochim Biophys Acta. 1958 Nov;30(2):324–328. doi: 10.1016/0006-3002(58)90057-x. [DOI] [PubMed] [Google Scholar]
  6. Szer W., Shugar D. The structure of poly-5-methylcytidylic acid and its twin-stranded complex with poly-inosinic acid. J Mol Biol. 1966 May;17(1):174–187. doi: 10.1016/s0022-2836(66)80102-x. [DOI] [PubMed] [Google Scholar]
  7. Todd Miles H., Frazier J. A strand disproportionation reaction in a helical polynucleotide system. Biochem Biophys Res Commun. 1964;14:129–136. doi: 10.1016/0006-291x(64)90242-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES