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. 1969 Aug;63(4):1128–1135. doi: 10.1073/pnas.63.4.1128

A NONDEFECTIVE (COMPETENT) ADENOVIRUS-SV40 HYBRID ISOLATED FROM THE AD.2-SV40 HYBRID POPULATION

Andrew M Lewis Jr 1,*, Myron J Levin 1,†, William H Wiese 1,*, Clyde S Crumpacker 1,*, Patrick H Henry 1,†
PMCID: PMC223439  PMID: 4311250

Abstract

A new nondefective hybrid virus has been plaque-isolated from the Ad.2-SV40 hybrid population. This virus replicates efficiently with one-hit kinetics in both human embryonic kidney and African green monkey kidney cells, induces an SV40 specific antigen which is detectable by immunofluorescence and complement-fixation using sera from SV40 tumor-bearing hamsters, and produces SV40-specific RNA detectable by DNA-RNA hybridization. The SV40-specific antigen induced by this virus is heat-stable, sensitive to inhibitors of DNA synthesis, serologically different from SV40 T and viral antigens, and is an unrecognized SV40 antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
  2. BLACK P. H., ROWE W. P. VIRAL STUDIES OF SV40 TUMORIGENESIS IN HAMSTERS. J Natl Cancer Inst. 1964 Jan;32:253–265. [PubMed] [Google Scholar]
  3. Baum S. G., Reich P. R., Hybner C. J., Rowe W. P., Weissman S. M. Biophysical evidence for linkage of adenovirus and SV40 DNA's in adenovirus 7-SV40 hybrid particles. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1509–1515. doi: 10.1073/pnas.56.5.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beardmore W. B., Havlick M. J., Serafini A., McLean I. W., Jr Interrelationship of adenovirus (type 4) and papovavirus (SV-40) in monkey kidney cell cultures. J Immunol. 1965 Sep;95(3):422–435. [PubMed] [Google Scholar]
  5. HUEBNER R. J., CHANOCK R. M., RUBIN B. A., CASEY M. J. INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUS. Proc Natl Acad Sci U S A. 1964 Dec;52:1333–1340. doi: 10.1073/pnas.52.6.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Levin M. J., Oxman M. N., Diamandopoulos G. T., Levine A. S., Henry P. H., Enders J. F. Virus-specific nucleic acids in SV40-exposed hamster embryo cell lines: correlation with S and T antigens. Proc Natl Acad Sci U S A. 1969 Feb;62(2):589–596. doi: 10.1073/pnas.62.2.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lewis A. M., Jr, Baum S. G., Prigge K. O., Rowe W. P. Occurrence of adenovirus-SV40 hybrids among monkey kidney cell adapted strains of adenovirus. Proc Soc Exp Biol Med. 1966 May;122(1):214–218. doi: 10.3181/00379727-122-31093. [DOI] [PubMed] [Google Scholar]
  8. Lewis A. M., Jr, Prigge K. O., Rowe W. P. Studies of adenovirus-SV40 hybrid viruses. IV. An adenovirus type 2 strain carrying the infectious SV40 genome. Proc Natl Acad Sci U S A. 1966 Mar;55(3):526–531. doi: 10.1073/pnas.55.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lewis A. M., Wiese W. H., Rowe W. P. The presence of antibodies in human serum to early (T) adenovirus antigens. Proc Natl Acad Sci U S A. 1967 Mar;57(3):622–629. doi: 10.1073/pnas.57.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oxman M. N., Rowe W. P., Black P. H. Studies of adenovirus-SV40 hybrid viruses, VI. Differential effects of interferon on SV40 and adenovirus T antigen formation in cells infected with SV40 virus, adenoviruses, and adenovirus-SV40 hybrid viruses. Proc Natl Acad Sci U S A. 1967 Apr;57(4):941–948. doi: 10.1073/pnas.57.4.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. RAPP F., MELNICK J. L., BUTEL J. S., KITAHARA T. THE INCORPORATION OF SV40 MATERIAL INTO ADENOVIRUS 7 AS MEASURED BY INTRANUCLEAR SYNTHESIS OF SV40 TUMOR ANTIGEN. Proc Natl Acad Sci U S A. 1964 Dec;52:1348–1352. doi: 10.1073/pnas.52.6.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rapp F., Butel J. S., Melnick J. L. SV40-adenovirus "hybrid" populations: transfer of SV40 determinants from one type of adenovirus to another. Proc Natl Acad Sci U S A. 1965 Sep;54(3):717–724. doi: 10.1073/pnas.54.3.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  15. Rowe W. P., Bau S. G. Studies of adenovirus SV40 hybrid viruses. II. Defectiveness of the hybrid particles. J Exp Med. 1965 Nov 1;122(5):955–966. doi: 10.1084/jem.122.5.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rowe W. P., Pugh W. E. Studies of adenovirus-SV40 hybrid viruses. V. Evidence for linkage between adenovirus and SV40 genetic materials. Proc Natl Acad Sci U S A. 1966 May;55(5):1126–1132. doi: 10.1073/pnas.55.5.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schell K., Lane W. T., Casey M. J., Huebner R. J. Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization. Proc Natl Acad Sci U S A. 1966 Jan;55(1):81–88. doi: 10.1073/pnas.55.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]

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