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. 1969 Apr;62(4):1137–1144. doi: 10.1073/pnas.62.4.1137

MULTIPLE PHENYLALANYL-TRANSFER RIBONUCLEIC ACID SYNTHETASE ACTIVITIES IN THE CYTOPLASM OF Neurospora crassa*

Fredrick J Kull 1,†, K Bruce Jacobson 1
PMCID: PMC223625  PMID: 5256412

Abstract

Several tRNA's specific for a particular amino acid have been shown to exist in multiple, or isoaccepting, forms. There is considerable interest in establishing whether multiple aminoacyl-tRNA synthetases also exist. We present evidence that the cytoplasm of Neurospora crassa contains three chromatographically separable phenylalanyl-tRNA synthetases distinct from mitochondrial phenylalanyl-tRNA synthetase. In addition to differences in chromatographic properties the three enzymes exhibit different affinities, in Tris-Cl buffer, toward purified species of valine and alanine tRNA's isolated from Escherichia coli. The two major chromatographic fractions have very similar sedimentation characteristics, which makes a monomer-dimer relationship unlikely.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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