Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Mar;62(3):899–905. doi: 10.1073/pnas.62.3.899

DIFFERENCES IN THE TRANSFER RNA'S OF NORMAL LIVER AND NOVIKOFF HEPATOMA*

B S Baliga 1,2, E Borek 1,2, I B Weinstein 1,2, P R Srinivasan 1,2
PMCID: PMC223683  PMID: 4308099

Abstract

—A comparison of the elution profiles of 18 aminoacyl-tRNA's from Novikoff hepatoma with those from normal liver on a methylated albuminkieselguhr column revealed the occurrence of new species of tRNA for histidine, tyrosine, and asparagine in the hepatoma. In addition, the hepatoma tRNA's for arginine, isoleucine, lysine, methionine, serine, alanine, and tryptophan eluted at a higher salt concentration than the corresponding tRNA's of normal liver. The remaining eight amino acids did not show any significant differences in the elution profiles.

Full text

PDF
901

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axel R., Weinstein B., Farber E. Patterns of transfer RNA in normal rat liver and during hepatic carcinogenesis. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1255–1260. doi: 10.1073/pnas.58.3.1255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERGQUIST P. L., MATTHEWS R. E. Occurrence and distribution of methylated purines in the ribonucleic acids of subcellular fractions. Biochem J. 1962 Nov;85:305–313. doi: 10.1042/bj0850305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doi R. H., Kaneko I., Goehler B. Regulation of a serine transfer RNA of Bacillus subtilis under two growth conditions. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1548–1551. doi: 10.1073/pnas.56.5.1548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fink L. M., Goto T. Rat liver phenylalanine tRNA: column purification and fluorescence studies. Biochem Biophys Res Commun. 1968 Sep 30;32(6):963–970. doi: 10.1016/0006-291x(68)90122-8. [DOI] [PubMed] [Google Scholar]
  5. Hung P. P., Overby L. R. Alteration in function of transfer ribonucleic acid of Escherichia coli after infection with phage Q beta. J Biol Chem. 1968 Nov 10;243(21):5525–5531. [PubMed] [Google Scholar]
  6. Kaneko I., Doi R. H. Alteration of valyl-sRNA during sporulation of bacillus subtilis. Proc Natl Acad Sci U S A. 1966 Mar;55(3):564–571. doi: 10.1073/pnas.55.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kano-Sueoka T., Sueoka N. Modification of leucyl-sRNA after bacteriophage infection. J Mol Biol. 1966 Sep;20(1):183–209. doi: 10.1016/0022-2836(66)90124-0. [DOI] [PubMed] [Google Scholar]
  8. Kelmers A. D., Novelli G. D., Stulberg M. P. Separation of transfer ribonucleic acids by reverse phase chromatography. J Biol Chem. 1965 Oct;240(10):3979–3983. [PubMed] [Google Scholar]
  9. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  10. Mittelman A., Hall R. H., Yohn D. S., Grace J. T., Jr The in vitro soluble RNA methylase activity of SV40-induced hamster tumors. Cancer Res. 1967 Aug;27(8):1409–1414. [PubMed] [Google Scholar]
  11. Revel M., Littauer U. Z. The isolation of methyl deficient phenylalanyl transfer RNA from E. coli. Biochem Biophys Res Commun. 1965 Jul 12;20(2):187–194. doi: 10.1016/0006-291x(65)90344-x. [DOI] [PubMed] [Google Scholar]
  12. STENT G. S. THE OPERON: ON ITS THIRD ANNIVERSARY. MODULATION OF TRANSFER RNA SPECIES CAN PROVIDE A WORKABLE MODEL OF AN OPERATOR-LESS OPERON. Science. 1964 May 15;144(3620):816–820. doi: 10.1126/science.144.3620.816. [DOI] [PubMed] [Google Scholar]
  13. Silber R., Goldstein B., Berman E., Decter J., Friend C. The effect of a murine leukemia virus on RNA metabolism. Cancer Res. 1967 Jul;27(7):1264–1269. [PubMed] [Google Scholar]
  14. Subak-Sharpe H., Shepherd W. M., Hay J. Studies on sRNA coded by herpes virus. Cold Spring Harb Symp Quant Biol. 1966;31:583–594. doi: 10.1101/sqb.1966.031.01.076. [DOI] [PubMed] [Google Scholar]
  15. Taylor M. W., Granger G. A., Buck C. A., Holland J. J. Similarities and differences among specific tRNA's in mammalian tissues. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1712–1719. doi: 10.1073/pnas.57.6.1712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsutsui E., Srinivasan P. R., Borek E. TRNA methylases in tumors of animal and human origin. Proc Natl Acad Sci U S A. 1966 Sep;56(3):1003–1009. doi: 10.1073/pnas.56.3.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Viale G. L., Fondelli Restelli A., Viale E. Basi metilate nei t-RNA dei tumori cerebrali. Tumori. 1967 Nov-Dec;53(6):533–539. doi: 10.1177/030089166705300603. [DOI] [PubMed] [Google Scholar]
  18. Wevers W. F., Baguley B. C., Ralph R. K. Comparison of transfer RNA and aminoacyl RNA ligases from calf liver and spleen. Biochim Biophys Acta. 1966 Sep;123(3):503–509. doi: 10.1016/0005-2787(66)90218-8. [DOI] [PubMed] [Google Scholar]
  19. Yang W. K., Novelli G. D. Isoaccepting +RNA's in mouse plasma cell tumors that synthesize different myeloma protein. Biochem Biophys Res Commun. 1968 May 23;31(4):534–539. doi: 10.1016/0006-291x(68)90510-x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES