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. 1994 Jun;112(3):615–622. doi: 10.1017/s0950268800051311

Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction.

H Wu 1, K Taniguchi 1, F Wakasugi 1, S Ukae 1, S Chiba 1, M Ohseto 1, A Hasegawa 1, T Urasawa 1, S Urasawa 1
PMCID: PMC2271515  PMID: 8005227

Abstract

The presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the four representative gene 4 alleles. Using the PCR, we surveyed the gene 4 alleles of 199 human rotaviruses in stools collected in Japan and Thailand. Strains with the gene 4 allele, corresponding to P1A serotype, were shown to be the most prevalent, but two strains with P2 gene 4 allele and one strain with P3 gene 4 allele were detected in Thailand and in Japan, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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