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. 1976 Aug;127(2):988–997. doi: 10.1128/jb.127.2.988-997.1976

Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.

M S Guyer, D Figurski, N Davidson
PMCID: PMC233009  PMID: 783125

Abstract

pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.

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Selected References

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