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. 1977 Apr;130(1):4–10. doi: 10.1128/jb.130.1.4-10.1977

Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants.

C B Bruni, V Colantuoni, L Sbordone, R Cortese, F Blasi
PMCID: PMC235167  PMID: 323237

Abstract

Escherichia coli K-12 hisT mutants were isolated, and their properties were studied. These mutants are derepressed for the histidine operon, map close to the purF locus at about 49.5 min on the E. coli linkage map, and lack pseudouridylate synthetase activity. The defect in this enzyme leads to the absence of pseudouridines in the anticodon loop of several transfer ribonucleic acid species, as evidenced by the altered elution profile on reversed-phase chromatography and resistance to amino acid analogues. Finally, the hisT mutants studied have a reduced growth rate that appears to be linked to hisT, although it is not known whether it is due to the same mutation. The normal generation time can be restored by supplementing the medium with adenine, uracil, and isoleucine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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