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. 1977 Jul;131(1):63–68. doi: 10.1128/jb.131.1.63-68.1977

Function of S-adenosylmethionine in germinating yeast ascospores.

S J Choih, A J Ferro, S K Shapiro
PMCID: PMC235391  PMID: 326770

Abstract

Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protein synthesis is initially required for the completion of germination in strain 4579. The inhibition of S-adenosylmethionine synthetase by trifluoromethionine prevents both germination and protein synthesis. During the initial stages of germination, the S-adenosylmethionine synthetase, S-adenosylmethionine decarboxylase, and transfer ribonucleic acid methyltransferases increased significantly, indicating that polyamines and/or the methylation of transfer ribonucleic acid are required for the initiation of germination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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