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. 1975 Dec;124(3):1077–1083. doi: 10.1128/jb.124.3.1077-1083.1975

Metabolism of phenol and cresols by Bacillus stearothermophilus.

J A Buswell
PMCID: PMC236008  PMID: 1194230

Abstract

An obligate thermophilic strain of Bacillus stearothermophilus, strain PH24, isolated from industrial sediment by elective culture, grew readily at 55 C on phenol or on one of the isomers of cresol as the major carbon source. Intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to oxidize, without lag, these substrates together with catechol, 3-methylcatechol, and 4-methylcatechol. Cell extracts prepared from B. stearothermophilus PH24 after growth in the presence of phenol converted phenol to catechol with a concomitant uptake of 1 mol of oxygen per mol of substrate in reaction mixtures supplemented with reduced nicotinamide adenine dinucleotide. These preparations also catalyzed the oxidation of o-cresol to 3-methylcatechol and of m-cresol and p-cresol to 4-methylcatechol. Enzyme activity was inhibited by 1 mM p-chloromercuribenzoate and by 0.1 mM 0-phenanthroline. Catechol and the corresponding methylcatechol intermediates were further dissimilated by cell extracts of phenol-grown cells via the meta-cleavage route to yield 2-hydroxymuconic semialdehyde and the respective methylated derivatives.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayly R. C., Dagley S., Gibson D. T. The metabolism of cresols by species of Pseudomonas. Biochem J. 1966 Nov;101(2):293–301. doi: 10.1042/bj1010293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayly R. C., Wigmore G. J. Metabolism of phenol and cresols by mutants of Pseudomonas putida. J Bacteriol. 1973 Mar;113(3):1112–1120. doi: 10.1128/jb.113.3.1112-1120.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bird J. A., Cain R. B. Microbial degradation of alkylbenzenesulphonates. Metabolism of homologues of short alkyl-chain length by an Alcaligenes sp. Biochem J. 1974 May;140(2):121–134. doi: 10.1042/bj1400121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buswell J. A. The meta-cleavage of catechol by a thermophilic Bacillus species. Biochem Biophys Res Commun. 1974 Oct 8;60(3):934–941. doi: 10.1016/0006-291x(74)90404-5. [DOI] [PubMed] [Google Scholar]
  5. Buswell J. A., Twomey D. G. Utilization of phenol and cresols by Bacillus stearothermophilus, strain PH24. J Gen Microbiol. 1975 Apr;87(2):377–379. doi: 10.1099/00221287-87-2-377. [DOI] [PubMed] [Google Scholar]
  6. Cain R. B., Bilton R. F., Darrah J. A. The metabolism of aromatic acids by micro-organisms. Metabolic pathways in the fungi. Biochem J. 1968 Aug;108(5):797–828. doi: 10.1042/bj1080797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crawford R. L. Novel pathway for degradation of protocatechuic acid in Bacillus species. J Bacteriol. 1975 Feb;121(2):531–536. doi: 10.1128/jb.121.2.531-536.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAGLEY S., GIBSON D. T. THE BACTERIAL DEGRADATION OF CATECHOL. Biochem J. 1965 May;95:466–474. doi: 10.1042/bj0950466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DAGLEY S., PATEL M. D. Oxidation of p-cresol and related compounds by a Pseudomonas. Biochem J. 1957 Jun;66(2):227–233. doi: 10.1042/bj0660227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dagley S. Catabolism of aromatic compounds by micro-organisms. Adv Microb Physiol. 1971;6(0):1–46. doi: 10.1016/s0065-2911(08)60066-1. [DOI] [PubMed] [Google Scholar]
  11. HENDERSON M. E. The metabolism of aromatic compounds related to lignin by some hyphomycetes and yeast-like fungi of soil. J Gen Microbiol. 1961 Sep;26:155–165. doi: 10.1099/00221287-26-1-155. [DOI] [PubMed] [Google Scholar]
  12. Hegeman G. D., Rosenberg S. L. The evolution of bacterial enzyme systems. Annu Rev Microbiol. 1970;24:429–462. doi: 10.1146/annurev.mi.24.100170.002241. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. NAKAGAWA H., TAKEDA Y. Phenol hydroxylase. Biochim Biophys Acta. 1962 Aug 13;62:423–426. doi: 10.1016/0006-3002(62)90275-5. [DOI] [PubMed] [Google Scholar]
  15. NOZAKI M., KAGAMIYAMA H., HAYAISHI O. METAPYROCATECHASE. I. PURIFICATION, CRYSTALLIZATION AND SOME PROPERTIES. Biochem Z. 1963;338:582–590. [PubMed] [Google Scholar]
  16. Nakazawa T., Yokota T. Benzoate metabolism in Pseudomonas putida(arvilla) mt-2: demonstration of two benzoate pathways. J Bacteriol. 1973 Jul;115(1):262–267. doi: 10.1128/jb.115.1.262-267.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Neujahr H. Y., Gaal A. Phenol hydroxylase from yeast. Purification and properties of the enzyme from Trichosporon cutaneum. Eur J Biochem. 1973 Jun;35(2):386–400. doi: 10.1111/j.1432-1033.1973.tb02851.x. [DOI] [PubMed] [Google Scholar]
  18. Neujahr H. Y., Lindsjö S., Varga J. M. Oxidation of phenols by cells and cell-free enzymes from Candida tropicalis. Antonie Van Leeuwenhoek. 1974;40(2):209–216. doi: 10.1007/BF00394378. [DOI] [PubMed] [Google Scholar]
  19. Neujahr H. Y., Varga J. M. Degradation of phenols by intact cells and cell-free preparations of Trichosporon cutaneum. Eur J Biochem. 1970 Mar 1;13(1):37–44. doi: 10.1111/j.1432-1033.1970.tb00896.x. [DOI] [PubMed] [Google Scholar]
  20. Ornston L. N., Stanier R. Y. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. J Biol Chem. 1966 Aug 25;241(16):3776–3786. [PubMed] [Google Scholar]
  21. Ribbons D. W. Metabolism of omicron-cresol by Pseudomonas aeruginosa strain T1. J Gen Microbiol. 1966 Aug;44(2):221–231. doi: 10.1099/00221287-44-2-221. [DOI] [PubMed] [Google Scholar]
  22. Sala-Trepat J. M., Evans W. C. The meta cleavage of catechol by Azotobacter species. 4-Oxalocrotonate pathway. Eur J Biochem. 1971 Jun 11;20(3):400–413. doi: 10.1111/j.1432-1033.1971.tb01406.x. [DOI] [PubMed] [Google Scholar]
  23. Willetts A. J., Cain R. B. Microbial metabolism of alkylbenzene sulphonates. Bacterial metabolism of undecylbenzene-p-sulphonate and dodecylbenzene-p-sulphonate. Biochem J. 1972 Sep;129(2):389–402. doi: 10.1042/bj1290389. [DOI] [PMC free article] [PubMed] [Google Scholar]

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