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. 1985 Jan;49(1):211–216. doi: 10.1128/aem.49.1.211-216.1985

Degradation of Dehydrodivanillin by Anaerobic Bacteria from Cow Rumen Fluid

Wei Chen 1, Kunio Ohmiya 1,*, Shoichi Shimizu 1, Hidekuni Kawakami 1
PMCID: PMC238372  PMID: 16346698

Abstract

Dehydrodivanillin (DDV; 0.15 g/liter) was biodegradable at 37°C under strictly anaerobic conditions by microflora from cow rumen fluid to the extent of 25% within 2 days in a yeast extract medium. The anaerobes were acclimated on DDV for 2 weeks, leading to DDV-degrading microflora with rates of degradation eight times higher than those initially. Dehydrodivanillic acid and vanillic acid were detected in an ethylacetate extract of a DDV-enriched culture broth by thin-layer, gas, and high-performance liquid chromatographies and by mass spectrometry.

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Selected References

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