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. 1989 Sep;63(9):4034–4038. doi: 10.1128/jvi.63.9.4034-4038.1989

Restricted growth of attenuated poliovirus strains in cultured cells of a human neuroblastoma.

V I Agol 1, S G Drozdov 1, T A Ivannikova 1, M S Kolesnikova 1, M B Korolev 1, E A Tolskaya 1
PMCID: PMC251001  PMID: 2548013

Abstract

Cultured cells of a human neuroblastoma, SK-N-MC, were found to be highly resistant to Sabin attenuated poliovirus types 1 and 2 strains; no appreciable cytopathic effect was observed, and the total harvest was generally in the order of 1 PFU per cell or less. On the other hand, related neurovirulent strains of these antigenic types produced a relatively good (2 orders of magnitude higher) yield in a markedly protracted infectious cycle. The limited growth of the attenuated virus in the neuroblastoma cells appeared to be confined to a minor cell subpopulation. Experiments with intratypic (type 1) poliovirus recombinants suggested that the major genetic determinants limiting reproduction of the attenuated polioviruses in the neuroblastoma cells are located in the 5' half of the viral RNA, although the 3' half also appears to contribute somewhat to this phenotype. The possibility that neuroblastoma cells may represent an in vitro model for studying poliovirus neurovirulence is briefly discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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