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. 1972 Dec;112(3):1177–1183. doi: 10.1128/jb.112.3.1177-1183.1972

Effect of Elevated Temperatures on Protein Synthesis in Escherichia coli

David Patterson a,1, David Gillespie b,2
PMCID: PMC251546  PMID: 4565533

Abstract

Upon a temperature shift from 30 C to 42 to 44 C, Escherichia coli experiences a transient slowdown in rate of optical density increase, uridine incorporation, and amino acid incorporation. The data show that the primary target for thermal inactivation is a molecule(s) required for the initiation of protein synthesis. The effect on ribonucleic acid synthesis is a secondary effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  2. Boyd D., Nixon R., Gillespie S., Gillespie D. Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation. J Bacteriol. 1968 Mar;95(3):1040–1050. doi: 10.1128/jb.95.3.1040-1050.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dube S. K., Rudland P. S. Control of translation by T4 phage: altered binding of disfavoured messengers. Nature. 1970 May 30;226(5248):820–823. doi: 10.1038/226820a0. [DOI] [PubMed] [Google Scholar]
  4. Gillespie S., Gillespie D. Ribonucleic acid-deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide. Biochem J. 1971 Nov;125(2):481–487. doi: 10.1042/bj1250481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Godson G. N., Sinsheimer R. L. Lysis of Escherichia coli with a neutral detergent. Biochim Biophys Acta. 1967 Dec 19;149(2):476–488. doi: 10.1016/0005-2787(67)90175-x. [DOI] [PubMed] [Google Scholar]
  6. Jacobson A., Gillespie D. Genetic complementation between Escherichia coli RNA polymerase mutants. Biochem Biophys Res Commun. 1971 Sep;44(5):1030–1040. doi: 10.1016/s0006-291x(71)80189-4. [DOI] [PubMed] [Google Scholar]
  7. Klem E. B., Hsu W. T., Weiss S. B. The selective inhibition of protein initiation by T4 phage-induced factors. Proc Natl Acad Sci U S A. 1970 Oct;67(2):696–701. doi: 10.1073/pnas.67.2.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lodish H. F. Bacteriophage f2 RNA: control of translation and gene order. Nature. 1968 Oct 26;220(5165):345–350. doi: 10.1038/220345a0. [DOI] [PubMed] [Google Scholar]
  10. Lodish H. F. Secondary structure of bacteriophage f2 ribonucleic acid and the initiation of in vitro protein biosynthesis. J Mol Biol. 1970 Jun 28;50(3):689–702. doi: 10.1016/0022-2836(70)90093-8. [DOI] [PubMed] [Google Scholar]
  11. McCormick W., Penman S. Regulation of protein synthesis in HeLa cells: translation at elevated temperatures. J Mol Biol. 1969 Jan;39(2):315–333. doi: 10.1016/0022-2836(69)90320-9. [DOI] [PubMed] [Google Scholar]
  12. Müller-Hill B., Crapo L., Gilbert W. Mutants that make more lac repressor. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259–1264. doi: 10.1073/pnas.59.4.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Patterson D., Gillespie D. Deductive analysis of a protein-synthesis mutant of Escherichia coli. Biochem Genet. 1973 Feb;8(2):205–230. doi: 10.1007/BF00485547. [DOI] [PubMed] [Google Scholar]
  14. Patterson D., Gillespie D. Stringent response of RNA synthesis in E. coli produced by a temperature shift-up. Biochem Biophys Res Commun. 1971 Oct 15;45(2):476–482. doi: 10.1016/0006-291x(71)90843-6. [DOI] [PubMed] [Google Scholar]
  15. Patterson D., Weinstein M., Marshall S., Gillespie D. A new RNA synthesis mutant of E. coli. Biochem Genet. 1971 Dec;5(6):563–578. doi: 10.1007/BF00485674. [DOI] [PubMed] [Google Scholar]
  16. Pollack Y., Groner Y., Aviv(Greenshpan) H., Revel M. Role of initiation factor B (F3) in the preferential translation of T4 late messenger RNA in T4 infected E. Coli. FEBS Lett. 1970 Aug 17;9(4):218–221. doi: 10.1016/0014-5793(70)80359-3. [DOI] [PubMed] [Google Scholar]
  17. Revel M., Herzberg M., Becarevic A., Gros F. Role of protein factor in the functional binding of ribosomes to natural messenger RNA. J Mol Biol. 1968 Apr 14;33(1):231–249. doi: 10.1016/0022-2836(68)90291-x. [DOI] [PubMed] [Google Scholar]
  18. Ron E. Z., Davis B. D. Growth rate of Escherichia coli at elevated temperatures: limitation by methionine. J Bacteriol. 1971 Aug;107(2):391–396. doi: 10.1128/jb.107.2.391-396.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ron E. Z., Shani M. Growth rate of Escherichia coli at elevated temperatures: reversible inhibition of homoserine trans-succinylase. J Bacteriol. 1971 Aug;107(2):397–400. doi: 10.1128/jb.107.2.397-400.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scheps R., Wax R., Revel M. Reactivation in vitro of inactive ribosomes from stationary phase Escherichia coli. Biochim Biophys Acta. 1971 Feb 25;232(1):140–150. doi: 10.1016/0005-2787(71)90498-9. [DOI] [PubMed] [Google Scholar]
  21. Shih A. Y., Eisenstadt J., Lengyel P. On the relation between ribonucleic acid synthesis and peptide chain initiation in E. coli. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1599–1605. doi: 10.1073/pnas.56.5.1599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Summers W. C. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate. Anal Biochem. 1970 Feb;33(2):459–463. doi: 10.1016/0003-2697(70)90316-7. [DOI] [PubMed] [Google Scholar]
  23. Young R. M., Nakada D. Defective ribosomes in chloramphenicol-treated Escherichia coli. J Mol Biol. 1971 May 14;57(3):457–473. doi: 10.1016/0022-2836(71)90103-3. [DOI] [PubMed] [Google Scholar]

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