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. 1968 Oct;96(4):1150–1158. doi: 10.1128/jb.96.4.1150-1158.1968

Effects of Ionizing Radiation on Synchronous Cultures of Escherichia coli B/r

D Joseph Clark a,1
PMCID: PMC252429  PMID: 4879555

Abstract

The sensitivity of Escherichia coli B/r to X-irradiation is correlated with the replication cycle of deoxyribonucleic acid (DNA). The sensitivity to X-irradiation in the wild type can be attributed to the presence of nuclear targets plus DNA repair mechanisms. The effects of nuclear targets are observed in the recombination-deficient (rec−) mutant B/r, but the sensitivity reflected by changes in the slope of killing curves is absent. A study of different growth conditions indicates that maximal resistance to X rays occurs toward the middle of the division cycle. Evidence is offered that branched chromosomes respond as one-hit targets to X-irradiation. The killing effects of heavy-ion bombardment on E. coli are due primarily to ionizing radiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER P., STACEY K. A. Cross-linking of deoxyribonucleic acid in sperm heads by ionizing radiations. Nature. 1959 Sep 26;184:958–960. doi: 10.1038/184958a0. [DOI] [PubMed] [Google Scholar]
  2. BILLEN D. UNBALANCED DEOXYRIBONUCLEIC ACID SYNTHESIS: ITS ROLE IN X-RAY-INDUCED BACTERIAL DEATH. Biochim Biophys Acta. 1963 Aug 20;72:608–618. [PubMed] [Google Scholar]
  3. CAIRNS J. The bacterial chromosome and its manner of replication as seen by autoradiography. J Mol Biol. 1963 Mar;6:208–213. doi: 10.1016/s0022-2836(63)80070-4. [DOI] [PubMed] [Google Scholar]
  4. Clark A. J. The beginning of a genetic analysis of recombination proficiency. J Cell Physiol. 1967 Oct;70(2 Suppl):165–180. doi: 10.1002/jcp.1040700412. [DOI] [PubMed] [Google Scholar]
  5. DEERING R. A. Studies on division inhibition and filament formation of Escherichia coli by ultraviolet light. J Bacteriol. 1958 Aug;76(2):123–130. doi: 10.1128/jb.76.2.123-130.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DEFILIPPES F. M., GUILD W. R. Irradiation of solutions of transforming DNA. Radiat Res. 1959 Jul;11(1):38–53. [PubMed] [Google Scholar]
  7. DREW R. M. Gamma-Irradiation of penumococcus deoxyribonucleic acid. Radiat Res. 1955 Oct;3(2):116–120. [PubMed] [Google Scholar]
  8. Donachie W. D. Control of enzyme steps during the bacterial cell cycle. Nature. 1965 Mar 13;205(976):1084–1086. doi: 10.1038/2051084a0. [DOI] [PubMed] [Google Scholar]
  9. Gorman J., Taruo P., LaBerge M., Halvorson H. Timing of enzyme synthesis during synchronous division in yeast. Biochem Biophys Res Commun. 1964 Feb 18;15(1):43–49. doi: 10.1016/0006-291x(64)90100-7. [DOI] [PubMed] [Google Scholar]
  10. HELMSTETTER C. E., CUMMINGS D. J. AN IMPROVED METHOD FOR THE SELECTION OF BACTERIAL CELLS AT DIVISION. Biochim Biophys Acta. 1964 Mar 16;82:608–610. doi: 10.1016/0304-4165(64)90453-2. [DOI] [PubMed] [Google Scholar]
  11. HELMSTETTER C. E., URETZ R. B. X-ray and ultraviolet sensitivity of synchronously dividing Escherichia coli. Biophys J. 1963 Jan;3:35–47. doi: 10.1016/s0006-3495(63)86802-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KAPLAN H. S., MOSES L. E. BIOLOGICAL COMPLEXITY AND RADIOSENSITIVITY. RADIATION LETHALITY IN CELLS AND VIRUSES IS CORRELATED WITH NUCLEIC ACID CONTENT, STRUCTURE, AND PLOIDY. Science. 1964 Jul 3;145(3627):21–25. doi: 10.1126/science.145.3627.21. [DOI] [PubMed] [Google Scholar]
  13. KAPLAN H. S., SMITH K. C., TOMLIN P. Radiosensitization of E. coli by purine and pyrimidine analogues incorporated in deoxyribonucleic acid. Nature. 1961 May 27;190:794–796. doi: 10.1038/190794a0. [DOI] [PubMed] [Google Scholar]
  14. KUEMPEL P. L., PARDEE A. B. THE CYCLE OF BACTERIAL DUPLICATION. J Cell Physiol. 1963 Oct;62:SUPPL1–SUPPL1:30. doi: 10.1002/jcp.1030620404. [DOI] [PubMed] [Google Scholar]
  15. Kaplan H. S. DNA-strand scission and loss of viability after x irradiation of normal and sensitized bacterial cells. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1442–1446. doi: 10.1073/pnas.55.6.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lark C. Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates. Biochim Biophys Acta. 1966 Jun 22;119(3):517–525. doi: 10.1016/0005-2787(66)90128-6. [DOI] [PubMed] [Google Scholar]
  17. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  19. MUNSON R. J., MACLEAN F. I. The nature and radiation sensitivity of the long forms of Escherichia coli strain B/r. J Gen Microbiol. 1961 May;25:29–39. doi: 10.1099/00221287-25-1-29. [DOI] [PubMed] [Google Scholar]
  20. Masters M., Kuempel P. L., Pardee A. B. Enzyme synthesis in synchronous cultures of bacteria. Biochem Biophys Res Commun. 1964 Feb 18;15(1):38–42. doi: 10.1016/0006-291x(64)90099-3. [DOI] [PubMed] [Google Scholar]
  21. OWEN M. E., MORTIMER R. K. Dominant lethality induced by x-rays in haploid and diploid Saccharomyces cerevisiae. Nature. 1956 Mar 31;177(4509):625–626. doi: 10.1038/177625b0. [DOI] [PubMed] [Google Scholar]
  22. Person S. Comparative Killing Efficiencies for Decays of Tritiated Compounds Incorporated into E. coli. Biophys J. 1963 May;3(3):183–187. doi: 10.1016/s0006-3495(63)86814-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SCHAECHTER M., WILLIAMSON J. P., HOOD J. R., Jr, KOCH A. L. Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962 Nov;29:421–434. doi: 10.1099/00221287-29-3-421. [DOI] [PubMed] [Google Scholar]
  24. STAPLETON G. E. Variations in the sensitivity of escherichia coli to ionizing radiations during the growth cycle. J Bacteriol. 1955 Oct;70(4):357–362. doi: 10.1128/jb.70.4.357-362.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WITKIN E. M. THE EFFECT OF ACRIFLAVINE ON PHOTOREVERSAL OF LETHAL AND MUTAGENIC DAMAGE PRODUCED IN BACTERIA BY ULTRAVIOLET LIGHT. Proc Natl Acad Sci U S A. 1963 Sep;50:425–430. doi: 10.1073/pnas.50.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. YANAGITA T., MARUYAMA Y., TAKEBE I. Cellular response to deleterious agents during the course of synchronous growth of Escherichia coli. J Bacteriol. 1958 May;75(5):523–529. doi: 10.1128/jb.75.5.523-529.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]

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