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. 1988 Aug;62(8):3068–3072. doi: 10.1128/jvi.62.8.3068-3072.1988

An internal 5'-noncoding region required for translation of poliovirus RNA in vitro.

K Bienkowska-Szewczyk 1, E Ehrenfeld 1
PMCID: PMC253752  PMID: 2839722

Abstract

A truncated poliovirus RNA that contains the entire 5'-noncoding region as well as some capsid protein-coding sequences was produced from cloned cDNA inserted into an SP6 transcription vector and subsequently was translated in a mixed rabbit reticulocyte-HeLa cell lysate. Deletions or modifications of regions of the 5'-noncoding sequences had significant effects upon the efficiency of translation. The presence of a 60-nucleotide sequence located at positions 567 to 627 appeared to be essential for active ribosome binding and translation of this uncapped RNA.

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Selected References

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