Abstract
The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Fluorescence intensity of potential-sensitive carbocyanine dyes was calibrated vs. electric membrane potential. Potassium and sodium ion diffusion potentials significantly contributed to mycoplasma membrane potential. Chloride ions were obviously freely permeable across mycoplasma membranes. Under growth conditions the mycoplasma membrane potential was estimated to be delta psi = - 80 mV.
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- Freedman J. C., Laris P. C. Electrophysiology of cells and organelles: studies with optical potentiometric indicators. Int Rev Cytol Suppl. 1981;12:177–246. doi: 10.1016/b978-0-12-364373-5.50015-9. [DOI] [PubMed] [Google Scholar]
- Harold F. M. Ion currents and physiological functions in microorganisms. Annu Rev Microbiol. 1977;31:181–203. doi: 10.1146/annurev.mi.31.100177.001145. [DOI] [PubMed] [Google Scholar]
- Pressman B. C. Biological applications of ionophores. Annu Rev Biochem. 1976;45:501–530. doi: 10.1146/annurev.bi.45.070176.002441. [DOI] [PubMed] [Google Scholar]
- Schiefer H. G., Schummer U. The electrochemical potential across mycoplasmal membranes. Rev Infect Dis. 1982 May-Jun;4 (Suppl):S65–S70. doi: 10.1093/clinids/4.supplement_1.s65. [DOI] [PubMed] [Google Scholar]
- Schummer U., Schiefer H. G. A novel method for the determination of electrical potentials across cellular membranes. I. Theoretical considerations and mathematical approach. Biochim Biophys Acta. 1980 Aug 14;600(3):993–997. doi: 10.1016/0005-2736(80)90501-5. [DOI] [PubMed] [Google Scholar]
- Schummer U., Schiefer H. G., Gerhardt U. A novel method for the determination of electrical potentials across cellular membranes. II. Membrane potentials of Acholeplasmas, Mycoplasmas, Streptococci and erythrocytes. Biochim Biophys Acta. 1980 Aug 14;600(3):998–106. doi: 10.1016/0005-2736(80)90502-7. [DOI] [PubMed] [Google Scholar]
- Schummer U., Schiefer H. G., Gerhardt U. Mycoplasma membrane potential determined by a fluorescent probe. Hoppe Seylers Z Physiol Chem. 1978 Aug;359(8):1023–1025. [PubMed] [Google Scholar]
- Waggoner A. S. Dye indicators of membrane potential. Annu Rev Biophys Bioeng. 1979;8:47–68. doi: 10.1146/annurev.bb.08.060179.000403. [DOI] [PubMed] [Google Scholar]