Skip to main content
The Yale Journal of Biology and Medicine logoLink to The Yale Journal of Biology and Medicine
. 1983 Sep-Dec;56(5-6):413–418.

Electrophysiology of mycoplasma membranes.

U Schummer, H G Schiefer
PMCID: PMC2590526  PMID: 6089453

Abstract

The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Fluorescence intensity of potential-sensitive carbocyanine dyes was calibrated vs. electric membrane potential. Potassium and sodium ion diffusion potentials significantly contributed to mycoplasma membrane potential. Chloride ions were obviously freely permeable across mycoplasma membranes. Under growth conditions the mycoplasma membrane potential was estimated to be delta psi = - 80 mV.

Full text

PDF
416

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Freedman J. C., Laris P. C. Electrophysiology of cells and organelles: studies with optical potentiometric indicators. Int Rev Cytol Suppl. 1981;12:177–246. doi: 10.1016/b978-0-12-364373-5.50015-9. [DOI] [PubMed] [Google Scholar]
  2. Harold F. M. Ion currents and physiological functions in microorganisms. Annu Rev Microbiol. 1977;31:181–203. doi: 10.1146/annurev.mi.31.100177.001145. [DOI] [PubMed] [Google Scholar]
  3. Pressman B. C. Biological applications of ionophores. Annu Rev Biochem. 1976;45:501–530. doi: 10.1146/annurev.bi.45.070176.002441. [DOI] [PubMed] [Google Scholar]
  4. Schiefer H. G., Schummer U. The electrochemical potential across mycoplasmal membranes. Rev Infect Dis. 1982 May-Jun;4 (Suppl):S65–S70. doi: 10.1093/clinids/4.supplement_1.s65. [DOI] [PubMed] [Google Scholar]
  5. Schummer U., Schiefer H. G. A novel method for the determination of electrical potentials across cellular membranes. I. Theoretical considerations and mathematical approach. Biochim Biophys Acta. 1980 Aug 14;600(3):993–997. doi: 10.1016/0005-2736(80)90501-5. [DOI] [PubMed] [Google Scholar]
  6. Schummer U., Schiefer H. G., Gerhardt U. A novel method for the determination of electrical potentials across cellular membranes. II. Membrane potentials of Acholeplasmas, Mycoplasmas, Streptococci and erythrocytes. Biochim Biophys Acta. 1980 Aug 14;600(3):998–106. doi: 10.1016/0005-2736(80)90502-7. [DOI] [PubMed] [Google Scholar]
  7. Schummer U., Schiefer H. G., Gerhardt U. Mycoplasma membrane potential determined by a fluorescent probe. Hoppe Seylers Z Physiol Chem. 1978 Aug;359(8):1023–1025. [PubMed] [Google Scholar]
  8. Waggoner A. S. Dye indicators of membrane potential. Annu Rev Biophys Bioeng. 1979;8:47–68. doi: 10.1146/annurev.bb.08.060179.000403. [DOI] [PubMed] [Google Scholar]

Articles from The Yale Journal of Biology and Medicine are provided here courtesy of Yale Journal of Biology and Medicine

RESOURCES