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. 1986 Jun;52(3):688–694. doi: 10.1128/iai.52.3.688-694.1986

Serum amyloid P-component-induced enhancement of macrophage listericidal activity.

P P Singh, F Gervais, E Skamene, R F Mortensen
PMCID: PMC260912  PMID: 3086231

Abstract

Purified serum amyloid P component (SAP), the major acute-phase reactant of mice, augmented the in vitro listericidal activity of inflammatory (elicited) macrophages, bone marrow-derived monocytes, and macrophages from a subcutaneous site of inflammation. Monocytes and macrophages from C57BL/B6 mice, which are relatively resistant to Listeria monocytogenes, exhibited a significantly greater enhanced killing capacity for listeria than macrophages from listeria-susceptible A/J mice. SAP did not alter the extent of phagocytosis by macrophages of opsonized L. monocytogenes, nor was SAP opsonic for listeria. Mannose-derived simple sugars inhibited the binding of SAP to macrophages and consequently prevented the enhanced SAP-dependent listericidal activity. Macrophages from lipopolysaccharide-hyporesponsive mice also had increased microbicidal activity following incubation with SAP. SAP activated macrophages independently of lymphokine. Therefore, SAP may serve as a mediator of the heightened nonspecific host defense response that is associated with the acute phase of the systemic inflammatory response.

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Selected References

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