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. 1986 Jun;52(3):688–694. doi: 10.1128/iai.52.3.688-694.1986

Serum amyloid P-component-induced enhancement of macrophage listericidal activity.

P P Singh, F Gervais, E Skamene, R F Mortensen
PMCID: PMC260912  PMID: 3086231

Abstract

Purified serum amyloid P component (SAP), the major acute-phase reactant of mice, augmented the in vitro listericidal activity of inflammatory (elicited) macrophages, bone marrow-derived monocytes, and macrophages from a subcutaneous site of inflammation. Monocytes and macrophages from C57BL/B6 mice, which are relatively resistant to Listeria monocytogenes, exhibited a significantly greater enhanced killing capacity for listeria than macrophages from listeria-susceptible A/J mice. SAP did not alter the extent of phagocytosis by macrophages of opsonized L. monocytogenes, nor was SAP opsonic for listeria. Mannose-derived simple sugars inhibited the binding of SAP to macrophages and consequently prevented the enhanced SAP-dependent listericidal activity. Macrophages from lipopolysaccharide-hyporesponsive mice also had increased microbicidal activity following incubation with SAP. SAP activated macrophages independently of lymphokine. Therefore, SAP may serve as a mediator of the heightened nonspecific host defense response that is associated with the acute phase of the systemic inflammatory response.

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Selected References

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  1. Barna B. P., Deodhar S. D., Gautam S., Yen-Lieberman B., Roberts D. Macrophage activation and generation of tumoricidal activity by liposome-associated human C-reactive protein. Cancer Res. 1984 Jan;44(1):305–310. [PubMed] [Google Scholar]
  2. Benson M. D., Skinner M., Shirahama T., Cohen A. S. P-component of amyloid. Isolation from human serum by affinity chromatography. Arthritis Rheum. 1976 Jul-Aug;19(4):749–754. doi: 10.1002/1529-0131(197607/08)19:4<749::aid-art1780190415>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  3. Cheers C., McKenzie I. F., Pavlov H., Waid C., York J. Resistance and susceptibility of mice to bacterial infection: course of listeriosis in resistant or susceptible mice. Infect Immun. 1978 Mar;19(3):763–770. doi: 10.1128/iai.19.3.763-770.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Czuprynski C. J., Henson P. M., Campbell P. A. Killing of Listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and nonimmune mice. J Leukoc Biol. 1984 Feb;35(2):193–208. doi: 10.1002/jlb.35.2.193. [DOI] [PubMed] [Google Scholar]
  5. Deodhar S. D., James K., Chiang T., Edinger M., Barna B. P. Inhibition of lung metastases in mice bearing a malignant fibrosarcoma by treatment with liposomes containing human C-reactive protein. Cancer Res. 1982 Dec;42(12):5084–5088. [PubMed] [Google Scholar]
  6. Ezekowitz R. A., Gordon S. Down-regulation of mannosyl receptor-mediated endocytosis and antigen F4/80 in bacillus Calmette-Guérin-activated mouse macrophages. Role of T lymphocytes and lymphokines. J Exp Med. 1982 Jun 1;155(6):1623–1637. doi: 10.1084/jem.155.6.1623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fauve R. M., Jusforgues H., Hevin B. Maintenance of granuloma macrophages in serum-free medium. J Immunol Methods. 1983 Nov 25;64(3):345–351. doi: 10.1016/0022-1759(83)90442-8. [DOI] [PubMed] [Google Scholar]
  8. Gervais F., Stevenson M., Skamene E. Genetic control of resistance to Listeria monocytogenes: regulation of leukocyte inflammatory responses by the Hc locus. J Immunol. 1984 Apr;132(4):2078–2083. [PubMed] [Google Scholar]
  9. Godfrey R. W., Horton P. G., Wilder M. S. Time course of antilisterial activity by immunologically activated murine peritoneal macrophages. Infect Immun. 1983 Feb;39(2):532–539. doi: 10.1128/iai.39.2.532-539.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hind C. R., Collins P. M., Renn D., Cook R. B., Caspi D., Baltz M. L., Pepys M. B. Binding specificity of serum amyloid P component for the pyruvate acetal of galactose. J Exp Med. 1984 Apr 1;159(4):1058–1069. doi: 10.1084/jem.159.4.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Horwitz M. A., Silverstein S. C. Influence of the Escherichia coli capsule on complement fixation and on phagocytosis and killing by human phagocytes. J Clin Invest. 1980 Jan;65(1):82–94. doi: 10.1172/JCI109663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones T., Youmans G. P. The in vitro inhibition of growth of intracellular Listeria monocytogenes by lymphocyte products. Cell Immunol. 1973 Dec;9(3):353–362. doi: 10.1016/0008-8749(73)90050-6. [DOI] [PubMed] [Google Scholar]
  13. KAPLOW L. S. SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965 Aug;26:215–219. [PubMed] [Google Scholar]
  14. Kilpatrick J. M., Volanakis J. E. Opsonic properties of C-reactive protein. Stimulation by phorbol myristate acetate enables human neutrophils to phagocytize C-reactive protein-coated cells. J Immunol. 1985 May;134(5):3364–3370. [PubMed] [Google Scholar]
  15. Kushner I., Gewurz H., Benson M. D. C-reactive protein and the acute-phase response. J Lab Clin Med. 1981 Jun;97(6):739–749. [PubMed] [Google Scholar]
  16. Le P. T., Mortensen R. F. In vitro induction of hepatocyte synthesis of the acute phase reactant mouse serum amyloid P-component by macrophages and IL 1. J Leukoc Biol. 1984 Jun;35(6):587–603. doi: 10.1002/jlb.35.6.587. [DOI] [PubMed] [Google Scholar]
  17. Le P. T., Muller M. T., Mortensen R. F. Acute phase reactants of mice. I. Isolation of serum amyloid P-component (SAP) and its induction by a monokine. J Immunol. 1982 Aug;129(2):665–672. [PubMed] [Google Scholar]
  18. Lepay D. A., Steinman R. M., Nathan C. F., Murray H. W., Cohn Z. A. Liver macrophages in murine listeriosis. Cell-mediated immunity is correlated with an influx of macrophages capable of generating reactive oxygen intermediates. J Exp Med. 1985 Jun 1;161(6):1503–1512. doi: 10.1084/jem.161.6.1503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Meltzer M. S., Occhionero M., Ruco L. P. Macrophage activation for tumor cytotoxicity: regulatory mechanisms for induction and control of cytotoxic activity. Fed Proc. 1982 Apr;41(6):2198–2205. [PubMed] [Google Scholar]
  20. Morrison D. C., Ryan J. L. Bacterial endotoxins and host immune responses. Adv Immunol. 1979;28:293–450. doi: 10.1016/s0065-2776(08)60802-0. [DOI] [PubMed] [Google Scholar]
  21. Mortensen R. F., Beisel K., Zeleznik N. J., Le P. T. Acute-phase reactants of mice. II. Strain dependence of serum amyloid P-component (SAP) levels and response to inflammation. J Immunol. 1983 Feb;130(2):885–889. [PubMed] [Google Scholar]
  22. North R. J. The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. J Exp Med. 1970 Sep 1;132(3):521–534. doi: 10.1084/jem.132.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Osmand A. P., Friedenson B., Gewurz H., Painter R. H., Hofmann T., Shelton E. Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins). Proc Natl Acad Sci U S A. 1977 Feb;74(2):739–743. doi: 10.1073/pnas.74.2.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pepys M. B., Baltz M. L. Acute phase proteins with special reference to C-reactive protein and related proteins (pentaxins) and serum amyloid A protein. Adv Immunol. 1983;34:141–212. doi: 10.1016/s0065-2776(08)60379-x. [DOI] [PubMed] [Google Scholar]
  25. Pepys M. B., Baltz M., Gomer K., Davies A. J., Doenhoff M. Serum amyloid P-component is an acute-phase reactant in the mouse. Nature. 1979 Mar 15;278(5701):259–261. doi: 10.1038/278259a0. [DOI] [PubMed] [Google Scholar]
  26. Pepys M. B., Dash A. C., Fletcher T. C., Richardson N., Munn E. A., Feinstein A. Analogues in other mammals and in fish of human plasma proteins, C-reactive protein and amyloid P component. Nature. 1978 May 11;273(5658):168–170. doi: 10.1038/273168a0. [DOI] [PubMed] [Google Scholar]
  27. Sarlo K. T., Mortensen R. F. Enhanced interleukin 1 (IL-1) production mediated by mouse serum amyloid P component. Cell Immunol. 1985 Jul;93(2):398–405. doi: 10.1016/0008-8749(85)90144-3. [DOI] [PubMed] [Google Scholar]
  28. Skamene E. Genetic regulation of host resistance to bacterial infection. Rev Infect Dis. 1983 Sep-Oct;5 (Suppl 4):S823–S832. doi: 10.1093/clinids/5.supplement_4.s823. [DOI] [PubMed] [Google Scholar]
  29. Stevenson M. M., Kongshavn P. A., Skamene E. Genetic linkage of resistance to Listeria monocytogenes with macrophage inflammatory responses. J Immunol. 1981 Aug;127(2):402–407. [PubMed] [Google Scholar]
  30. Warren M. K., Vogel S. N. Bone marrow-derived macrophages: development and regulation of differentiation markers by colony-stimulating factor and interferons. J Immunol. 1985 Feb;134(2):982–989. [PubMed] [Google Scholar]
  31. Wing E. J., Gardner I. D., Ryning F. W., Remington J. S. Dissociation of effector functions in populations of activated macrophages. Nature. 1977 Aug 18;268(5621):642–644. doi: 10.1038/268642a0. [DOI] [PubMed] [Google Scholar]
  32. Wright S. D., Craigmyle L. S., Silverstein S. C. Fibronectin and serum amyloid P component stimulate C3b- and C3bi-mediated phagocytosis in cultured human monocytes. J Exp Med. 1983 Oct 1;158(4):1338–1343. doi: 10.1084/jem.158.4.1338. [DOI] [PMC free article] [PubMed] [Google Scholar]

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