Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Oct;18(4):825–829. doi: 10.1128/jcm.18.4.825-829.1983

Hydrogenase activity in catalase-positive strains of Campylobacter spp.

T G Goodman, P S Hoffman
PMCID: PMC270913  PMID: 6355162

Abstract

A rapid hydrogenase assay has been developed which may be useful in separating the species Campylobacter jejuni and C. coli from the subspecies C. fetus subsp. fetus and C. fetus subsp. venerealis. This assay employs the impermeant redox dye benzyl viologen, and positive determinations can be made within 20 min. All strains of C. jejuni and C. coli were found to be strongly hydrogenase positive. All strains of C. fetus subsp. fetus and C. fetus subsp. venerealis were negative for hydrogenase when the assay was performed at a benzyl viologen concentration of 2 mM and an incubation temperature of 30 degrees C. Some strains of C. fetus had low levels of hydrogenase as determined with cell extracts but were hydrogenase negative by the benzyl viologen assay. Since there are few rapid diagnostic tests available for screening Campylobacter isolates, we hope that the rapid hydrogenase assay will prove useful.

Full text

PDF
826

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaser M. J., Berkowitz I. D., LaForce F. M., Cravens J., Reller L. B., Wang W. L. Campylobacter enteritis: clinical and epidemiologic features. Ann Intern Med. 1979 Aug;91(2):179–185. doi: 10.7326/0003-4819-91-2-179. [DOI] [PubMed] [Google Scholar]
  2. Harvey S. M. Hippurate hydrolysis by Campylobacter fetus. J Clin Microbiol. 1980 Apr;11(4):435–437. doi: 10.1128/jcm.11.4.435-437.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hoffman P. S., Goodman T. G. Respiratory physiology and energy conservation efficiency of Campylobacter jejuni. J Bacteriol. 1982 Apr;150(1):319–326. doi: 10.1128/jb.150.1.319-326.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hwang M. N., Ederer G. M. Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J Clin Microbiol. 1975 Jan;1(1):114–115. doi: 10.1128/jcm.1.1.114-115.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hébert G. A., Hollis D. G., Weaver R. E., Lambert M. A., Blaser M. J., Moss C. W. 30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni. J Clin Microbiol. 1982 Jun;15(6):1065–1073. doi: 10.1128/jcm.15.6.1065-1073.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Janssen D., Helstad A. G. Isolation of Campylobacter fetus subsp. jejuni from human fecal specimens by incubation at 35 and 42 degrees C. J Clin Microbiol. 1982 Aug;16(2):398–399. doi: 10.1128/jcm.16.2.398-399.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kröger A., Dorrer E., Winkler E. The orientation of the substrate sites of formate dehydrogenase and fumarate reductase in the membrane of Vibrio succinogenes. Biochim Biophys Acta. 1980 Jan 4;589(1):118–136. doi: 10.1016/0005-2728(80)90136-x. [DOI] [PubMed] [Google Scholar]
  8. Odom J. M., Peck H. D., Jr Localization of dehydrogenases, reductases, and electron transfer components in the sulfate-reducing bacterium Desulfovibrio gigas. J Bacteriol. 1981 Jul;147(1):161–169. doi: 10.1128/jb.147.1.161-169.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Payne W. J., Grant M. A., Shapleigh J., Hoffman P. Nitrogen oxide reduction in Wolinella succinogenes and Campylobacter species. J Bacteriol. 1982 Nov;152(2):915–918. doi: 10.1128/jb.152.2.915-918.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Razi M. H., Park R. W., Skirrow M. B. Two new tests for differentiating between strains of Campylobacter. J Appl Bacteriol. 1981 Feb;50(1):55–57. doi: 10.1111/j.1365-2672.1981.tb00870.x. [DOI] [PubMed] [Google Scholar]
  11. Skirrow M. B., Benjamin J. '1001' Campylobacters: cultural characteristics of intestinal campylobacters from man and animals. J Hyg (Lond) 1980 Dec;85(3):427–442. doi: 10.1017/s0022172400063506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smibert R. M. The genus Campylobacter. Annu Rev Microbiol. 1978;32:673–709. doi: 10.1146/annurev.mi.32.100178.003325. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES