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. 1983 Oct;18(4):825–829. doi: 10.1128/jcm.18.4.825-829.1983

Hydrogenase activity in catalase-positive strains of Campylobacter spp.

T G Goodman, P S Hoffman
PMCID: PMC270913  PMID: 6355162

Abstract

A rapid hydrogenase assay has been developed which may be useful in separating the species Campylobacter jejuni and C. coli from the subspecies C. fetus subsp. fetus and C. fetus subsp. venerealis. This assay employs the impermeant redox dye benzyl viologen, and positive determinations can be made within 20 min. All strains of C. jejuni and C. coli were found to be strongly hydrogenase positive. All strains of C. fetus subsp. fetus and C. fetus subsp. venerealis were negative for hydrogenase when the assay was performed at a benzyl viologen concentration of 2 mM and an incubation temperature of 30 degrees C. Some strains of C. fetus had low levels of hydrogenase as determined with cell extracts but were hydrogenase negative by the benzyl viologen assay. Since there are few rapid diagnostic tests available for screening Campylobacter isolates, we hope that the rapid hydrogenase assay will prove useful.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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