Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Apr;11(4):363–369. doi: 10.1128/jcm.11.4.363-369.1980

WSJM, a simple chemically defined medium for growth of Neisseria gonorrhoeae.

T P Wong, R K Shockley, K H Johnston
PMCID: PMC273405  PMID: 6768768

Abstract

The growth requirements of 135 clinical isolates of Neisseria gonorrhoeae and six American Type Culture Collection reference strains were examined by using a simple chemically defined medium known as Wong-Shockley-Johnston medium, WSJM. The simple liquid medium supported growth of gonococci from an inoculum of 2 X 10(6) colony-forming units ml-1 and yielded 10(10) colony-forming units ml-1 in 10 h in the absence of CO2. Scale-up experiments with the complete medium yielded 5 to 10 g, wet weight, of cells per liter. The complete medium was stable upon storage at 5 degrees C and after lyophilization.

Full text

PDF
366

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  3. Catlin B. W., Pace P. J. Auxotypes and penicillin susceptibilities of Neisseria gonorrhoeae isolated from patients with gonorrhea involving two or more sites. Antimicrob Agents Chemother. 1977 Aug;12(2):147–156. doi: 10.1128/aac.12.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catlin B. W., Pace P. J. Nutritional requirements and penicillin susceptibilities of gonococci from pharyngeal and anogenital sites. Br J Vener Dis. 1977 Oct;53(5):299–303. doi: 10.1136/sti.53.5.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crawford C., Knapp J. S., Hale J., Holmes K. K. Asymptomatic gonorrhea in men: caused by gonococci with unique nutritional requirements. Science. 1977 Jun 17;196(4296):1352–1353. doi: 10.1126/science.405742. [DOI] [PubMed] [Google Scholar]
  6. Elmros T., Sandström G., Burman L. Autolysis of Neisseria gonorrhoeae. Relation between mechanical stability and viability. Br J Vener Dis. 1976 Aug;52(4):246–249. doi: 10.1136/sti.52.4.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hendry A. T., Stewart I. O. Auxanographic grouping and typing of Neisseria gonorrhoeae. Can J Microbiol. 1979 Apr;25(4):512–521. doi: 10.1139/m79-075. [DOI] [PubMed] [Google Scholar]
  8. Hunter K. M., McVeigh I. Development of a chemically defined medium for growth of Neisseria gonorrhoeae. Antonie Van Leeuwenhoek. 1970;36(2):305–316. doi: 10.1007/BF02069032. [DOI] [PubMed] [Google Scholar]
  9. Jones R. T., Talley R. S. Effects of Gaseous CO2 and bicarbonate on the growth of Neisseria gonorrhoeae. J Clin Microbiol. 1977 Apr;5(4):427–432. doi: 10.1128/jcm.5.4.427-432.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  12. La Scolea L. J., Jr, Young F. E. Development of a defined minimal medium for the growth of Neisseria gonorrhoeae. Appl Microbiol. 1974 Jul;28(1):70–76. doi: 10.1128/am.28.1.70-76.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morello J. A., Lerner S. A., Bohnhoff M. Characteristics of atypical Neisseria gonorrhoeae from disseminated and localized infections. Infect Immun. 1976 May;13(5):1510–1516. doi: 10.1128/iai.13.5.1510-1516.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Short H. B., Ploscowe V. B., Weiss J. A., Young F. E. Rapid method for auxotyping multiple strains of Neisseria gonorrhoeae. J Clin Microbiol. 1977 Sep;6(3):244–248. doi: 10.1128/jcm.6.3.244-248.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wood D. O., Brownell G. H. Transformation of leucine and rifampin traits in Neisseria gonorrhoeae with deoxyribonucleic acid from homologous and heterologous origins. J Bacteriol. 1975 Feb;121(2):471–474. doi: 10.1128/jb.121.2.471-474.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES