Abstract
During a 12-month period, 19,457 blood cultures were collected. Yeasts were isolated from 193 cultures derived from 76 cancer patients. Candida albicans or Candida tropicalis accounted for 79% of isolates. Of the three methods compared, the radiometric method required 2.9 days to become positive, "blind" subculture required 2.6 days, and Gram stains required 1 day. However, the radiometric method was clearly superior in detecting positive cultures, since 73% of all cultures were first detected radiometrically, 22% were detected by subculture, and only 5% were detected by Gram stain. Although 93% of the isolates were detected by aerobic culture, five (7%) isolates were obtained only from anaerobic cultures. Seven days of incubation appear to be sufficient for the radiometric detection of yeasts.
Full text
PDF![329](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c0d/273585/9bef6493c00a/jcm00170-0055.png)
![330](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c0d/273585/b1dd3d77fc03/jcm00170-0056.png)
![331](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c0d/273585/5d9e13141626/jcm00170-0057.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blazevic D. J., Stemper J. E., Matsen J. M. Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures. J Clin Microbiol. 1975 Feb;1(2):154–156. doi: 10.1128/jcm.1.2.154-156.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bodey G. P. Fungal infections complicating acute leukemia. J Chronic Dis. 1966 Jun;19(6):667–687. doi: 10.1016/0021-9681(66)90066-x. [DOI] [PubMed] [Google Scholar]
- Brooks K., Sodeman T. Rapid detection of bacteremia by a radiometric system. A clinical evaluation. Am J Clin Pathol. 1974 Jun;61(6):859–866. doi: 10.1093/ajcp/61.6.859. [DOI] [PubMed] [Google Scholar]
- Coleman R. M., Laslie W. W., Lambe D. W., Jr Clinical comparison of aerobic, hypertonic, and anaerobic culture media for the radiometric detection of bacteremia. J Clin Microbiol. 1976 Mar;3(3):281–286. doi: 10.1128/jcm.3.3.281-286.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeBlanc H. J., Jr, DeLand F., Wagner H. N., Jr Automated radiometric detection of bacteria in 2,967 blood cultures. Appl Microbiol. 1971 Nov;22(5):846–849. doi: 10.1128/am.22.5.846-849.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gröschel D., Hopfer R. L., French J. E. Blood cultures with the BACTEC 225 radiometric microbial growth detection system. Zentralbl Bakteriol Orig A. 1979 Jul;244(2-3):316–323. [PubMed] [Google Scholar]
- Harkness J. L., Hall M., Ilstrup D. M., Washington J. A., 2nd Effects of atmosphere of incubation and of routine subcultures on detection of bacteremia in vacuum blood culture bottles. J Clin Microbiol. 1975 Oct;2(4):296–299. doi: 10.1128/jcm.2.4.296-299.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts G. D., Washington J. A., 2nd Detection of fungi in blood cultures. J Clin Microbiol. 1975 Mar;1(3):309–310. doi: 10.1128/jcm.1.3.309-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]