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. 1980 Sep;12(3):329–331. doi: 10.1128/jcm.12.3.329-331.1980

Radiometric detection of yeasts in blood cultures of cancer patients.

R L Hopfer, A Orengo, S Chesnut, M Wenglar
PMCID: PMC273585  PMID: 7012168

Abstract

During a 12-month period, 19,457 blood cultures were collected. Yeasts were isolated from 193 cultures derived from 76 cancer patients. Candida albicans or Candida tropicalis accounted for 79% of isolates. Of the three methods compared, the radiometric method required 2.9 days to become positive, "blind" subculture required 2.6 days, and Gram stains required 1 day. However, the radiometric method was clearly superior in detecting positive cultures, since 73% of all cultures were first detected radiometrically, 22% were detected by subculture, and only 5% were detected by Gram stain. Although 93% of the isolates were detected by aerobic culture, five (7%) isolates were obtained only from anaerobic cultures. Seven days of incubation appear to be sufficient for the radiometric detection of yeasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blazevic D. J., Stemper J. E., Matsen J. M. Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures. J Clin Microbiol. 1975 Feb;1(2):154–156. doi: 10.1128/jcm.1.2.154-156.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bodey G. P. Fungal infections complicating acute leukemia. J Chronic Dis. 1966 Jun;19(6):667–687. doi: 10.1016/0021-9681(66)90066-x. [DOI] [PubMed] [Google Scholar]
  3. Brooks K., Sodeman T. Rapid detection of bacteremia by a radiometric system. A clinical evaluation. Am J Clin Pathol. 1974 Jun;61(6):859–866. doi: 10.1093/ajcp/61.6.859. [DOI] [PubMed] [Google Scholar]
  4. Coleman R. M., Laslie W. W., Lambe D. W., Jr Clinical comparison of aerobic, hypertonic, and anaerobic culture media for the radiometric detection of bacteremia. J Clin Microbiol. 1976 Mar;3(3):281–286. doi: 10.1128/jcm.3.3.281-286.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DeBlanc H. J., Jr, DeLand F., Wagner H. N., Jr Automated radiometric detection of bacteria in 2,967 blood cultures. Appl Microbiol. 1971 Nov;22(5):846–849. doi: 10.1128/am.22.5.846-849.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gröschel D., Hopfer R. L., French J. E. Blood cultures with the BACTEC 225 radiometric microbial growth detection system. Zentralbl Bakteriol Orig A. 1979 Jul;244(2-3):316–323. [PubMed] [Google Scholar]
  7. Harkness J. L., Hall M., Ilstrup D. M., Washington J. A., 2nd Effects of atmosphere of incubation and of routine subcultures on detection of bacteremia in vacuum blood culture bottles. J Clin Microbiol. 1975 Oct;2(4):296–299. doi: 10.1128/jcm.2.4.296-299.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Roberts G. D., Washington J. A., 2nd Detection of fungi in blood cultures. J Clin Microbiol. 1975 Mar;1(3):309–310. doi: 10.1128/jcm.1.3.309-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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