Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Jul;92(1):49–55. doi: 10.1128/jb.92.1.49-55.1966

Inhibition of Protein Synthesis by Spermine in Growing Cells of Staphylococcus aureus

Mischa E Friedman a,1, Uriel Bachrach a
PMCID: PMC276194  PMID: 5941283

Abstract

Friedman, Mischa E. (Hebrew University, Jerusalem, Israel), and Uriel Bachrach. Inhibition of protein synthesis by spermine in growing cells of Staphylococcus aureus. J. Bacteriol. 92:49–55. 1966.—Staphylococcus aureus SFL 9725 incorporated valine-C14 into cellular protein. This incorporation was inhibited by spermine when the pH of the culture was adjusted to 7.8, and the inhibition was antagonized partially by Mg++. The incorporation of C14-labeled leucine, phenylalanine, lysine, arginine, and possibly glutamic acid was inhibited to a much greater extent than that of alanine, glycine, or threonine. The uptake of spermine-C14 by S. aureus cells was rapid. More than 50% of the radioactivity resided in the soluble extract. The protein fraction of the soluble extract contained 99% of the recovered label, whereas the ribonucleic acid and deoxyribonucleic acid fractions contained little or no spermine-C14.

Full text

PDF
51

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACHRACH U., PERSKY S. ANTIBACTERIAL ACTION OF OXIDIZED SPERMINE. J Gen Microbiol. 1964 Nov;37:195–204. doi: 10.1099/00221287-37-2-195. [DOI] [PubMed] [Google Scholar]
  2. BAIRD-PARKER A. C. THE CLASSIFICATION OF STAPHYLOCOCCI AND MICROCOCCI FROM WORLD-WIDE SOURCES. J Gen Microbiol. 1965 Mar;38:363–387. doi: 10.1099/00221287-38-3-363. [DOI] [PubMed] [Google Scholar]
  3. COHEN S. S., LICHTENSTEIN J. Polyamines and ribosome structure. J Biol Chem. 1960 Jul;235:2112–2116. [PubMed] [Google Scholar]
  4. DAVIDSON J. D., FEIGELSON P. Practical aspects of internal-sample liquid-scintillation counting. Int J Appl Radiat Isot. 1957 Apr;2(1):1–18. doi: 10.1016/0020-708x(57)90021-2. [DOI] [PubMed] [Google Scholar]
  5. Davis B. D. The Isolation of Biochemically Deficient Mutants of Bacteria by Means of Penicillin. Proc Natl Acad Sci U S A. 1949 Jan;35(1):1–10. doi: 10.1073/pnas.35.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FELSENFELD G., HUANG S. The interaction of polynucleotides with metal ions, amino acids, and polyamines. Biochim Biophys Acta. 1960 Jan 29;37:425–433. doi: 10.1016/0006-3002(60)90498-4. [DOI] [PubMed] [Google Scholar]
  7. FRIEDMAN S. M., WEINSTEIN I. B. LACK OF FIDELITY IN THE TRANSLATION OF SYNTHETIC POLYRIBONUCLEOTIDES. Proc Natl Acad Sci U S A. 1964 Oct;52:988–996. doi: 10.1073/pnas.52.4.988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HERBST E. J., SNELL E. E. Putrescine and related compounds as growth factors for Hemophilus parainfluenzae 7991. J Biol Chem. 1949 Nov;181(1):47–54. [PubMed] [Google Scholar]
  9. LEVINTHAL C., KEYNAN A., HIGA A. Messenger RNA turnover and protein synthesis in B. subtilis inhibited by actinomycin D. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1631–1638. doi: 10.1073/pnas.48.9.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MAGER J., BENEDICT M., ARTMAN M. A common site of action of polyamines and streptomycin. Biochim Biophys Acta. 1962 Jul 30;62:202–204. doi: 10.1016/0006-3002(62)90519-x. [DOI] [PubMed] [Google Scholar]
  11. MAGER J. The stabilizing effect of spermine and related polyamines and bacterial protoplasts. Biochim Biophys Acta. 1959 Dec;36:529–531. doi: 10.1016/0006-3002(59)90195-7. [DOI] [PubMed] [Google Scholar]
  12. MAHLER H. R., MEHROTRA B. D. Dependence of deoxyribonucleic-amine interactions on deoxyribonucleic acid composition. Biochim Biophys Acta. 1962 Jan 22;55:252–254. doi: 10.1016/0006-3002(62)90965-4. [DOI] [PubMed] [Google Scholar]
  13. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  14. MATTHAEI H., NIRENBERG M. W. The dependence of cell-free protein synthesis in E. coli upon RNA prepared from ribosomes. Biochem Biophys Res Commun. 1961 Apr 28;4:404–408. doi: 10.1016/0006-291x(61)90298-4. [DOI] [PubMed] [Google Scholar]
  15. Martin R. G., Ames B. N. THE EFFECT OF POLYAMINES AND OF POLY U SIZE ON PHENYLALANINE INCORPORATION. Proc Natl Acad Sci U S A. 1962 Dec;48(12):2171–2178. doi: 10.1073/pnas.48.12.2171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nirenberg M., Leder P., Bernfield M., Brimacombe R., Trupin J., Rottman F., O'Neal C. RNA codewords and protein synthesis, VII. On the general nature of the RNA code. Proc Natl Acad Sci U S A. 1965 May;53(5):1161–1168. doi: 10.1073/pnas.53.5.1161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PARK J. T., HANCOCK R. A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. J Gen Microbiol. 1960 Feb;22:249–258. doi: 10.1099/00221287-22-1-249. [DOI] [PubMed] [Google Scholar]
  18. RAZIN S., ROZANSKY R. Mechanism of the antibacterial action of spermine. Arch Biochem Biophys. 1959 Mar;81(1):36–54. doi: 10.1016/0003-9861(59)90173-0. [DOI] [PubMed] [Google Scholar]
  19. ROSENTHAL S. M., DUBIN D. T. Metabolism of polyamines by Staphylococcus. J Bacteriol. 1962 Oct;84:859–863. doi: 10.1128/jb.84.4.859-863.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. ROZANSKY R., BACHRACH U., GROSSOWICZ N. Studies on the antibacterial action of spermine. J Gen Microbiol. 1954 Feb;10(1):11–16. doi: 10.1099/00221287-10-1-11. [DOI] [PubMed] [Google Scholar]
  21. SEVAG M. G., DRABBLE W. T. Prevention of the emergence of drug-resistant bacteria by polyamines. Biochem Biophys Res Commun. 1962 Aug 31;8:446–452. doi: 10.1016/0006-291x(62)90294-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES