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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jun;66(2):377–384. doi: 10.1073/pnas.66.2.377

Isolation of Temperature-Sensitive Mutants of L-Cells*

L H Thompson 1,2,3,, R Mankovitz 1,2,3,, R M Baker 1,2,3,§, J E Till 1,2,3, L Siminovitch 1,2,3, G F Whitmore 1,2,3
PMCID: PMC283055  PMID: 5271170

Abstract

Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine.

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Selected References

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