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. 1970 Oct;67(2):1034–1041. doi: 10.1073/pnas.67.2.1034

DNA Synthesis by RNA-Containing Tumor Viruses

Edward M Scolnick 1, Stuart A Aaronson 1, George J Todaro 1
PMCID: PMC283309  PMID: 4331715

Abstract

Murine leukemia (Rauscher and Moloney strains) and sarcoma (Kirsten strain) virions, as well as the mammary tumor virus of mice, contain an RNA-dependent DNA polymerase. Optimal incorporation of deoxyribonucleoside triphosphates occurs at a critical detergent (Triton X-100) concentration (0.010-0.014%). At higher than optimal detergent concentrations the virion is seen to be disrupted and enzyme activity is lost. The virion, enzymatic activity, and newly synthesized DNA all cosediment in a sucrose gradient. Thus far the enzymatic activity has been found only in RNA viruses that have oncogenic properties.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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