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. 1963 Dec;86(6):1202–1210. doi: 10.1128/jb.86.6.1202-1210.1963

DE NOVO SYNTHESIS OF α-AMYLASE BY BACILLUS STEAROTHERMOPHILUS1

N E Welker a,2, L Leon Campbell b,2
PMCID: PMC283630  PMID: 14086090

Abstract

Welker, N. E. (Western Reserve University, Cleveland, Ohio and University of Illinois, Urbana), and L. Leon Campbell. De novo synthesis of α-amylase by Bacillus stearothermophilus. J. Bacteriol. 86:1202–1210. 1963.—The pH optimum for the synthesis of α-amylase by washed-cell suspensions was 6.7. α-Amylase synthesis began soon after the addition of the inducer (maltose, methyl-β-d-maltoside, or phenyl-α-d-glucoside, at 10−3m), proceeded at a linear rate for 60 min, and then leveled off. Cell suspensions without inducer produced small amounts of α-amylase. The addition of glucose (2 × 10−3m), sucrose (10−3m), or glycerol (4 × 10−3m) to washed-cell suspensions failed to stimulate the production of α-amylase. Nitrogen starvation of washed cells for 60 min with fructose as a carbon source or by induction with pure maltose showed that the ability to produce α-amylase was lost. Examination of the amino acid pool at this time showed a general depletion of amino acids and the complete disappearance of tyrosine, phenyl-alanine, proline, and valine. Replenishment of the amino acid pool with casein hydrolysate (0.5%) restored the ability of the cells to produce α-amylase. Chloramphenicol and 8-azaguanine were shown to inhibit α-amylase synthesis. Inhibition was observed immediately upon the addition of chloramphenicol to cell suspensions preinduced for varying periods of time. Actinomycin D and mitomycin C also inhibited α-amylase synthesis when added to induced washed-cell suspensions. The amino acid analogues, norvaline, norleucine, and ethionine, inhibited α-amylase formation by 72, 53, and 38%, respectively. p-Fluorophenylalanine inhibited the synthesis of active α-amylase by 92% and the incorporation of proline-C14 into α-amylase and cellular proteins by 95 and 74%, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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