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. 1970 Apr;102(1):85–96. doi: 10.1128/jb.102.1.85-96.1970

d-Glucaric Acid and Galactaric Acid Catabolism by Agrobacterium tumefaciens

Yung Feng Chang a,1, David Sidney Feingold a
PMCID: PMC284973  PMID: 4314480

Abstract

Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to α-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to α-ketoglutaric acid. Since A. tumefaciens converts d-glucuronic acid to d-glucarate, a pathway from d-glucuronate to α-ketoglutarate in A. tumefaciens was determined.

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Selected References

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