Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Nov;116(2):511–516. doi: 10.1128/jb.116.2.511-516.1973

Isolation and Characterization of φ80 Transducing Bacteriophage for a Ribonucleic Acid Polymerase Gene

Bruce Konrad a,1, Joel Kirschbaum a, Stuart Austin a
PMCID: PMC285411  PMID: 4583236

Abstract

A new method for isolating specialized transducing phages is described. It was used to isolate a group of φ80 transducing phages which carry various bacterial markers from the metB region of the Escherichia coli chromosome. Some of the phages selected for transduction of the supA36 marker were also shown to carry rif, a locus known to specify the β subunit of ribonucleic acid polymerase. Expression of the prophage rifr gene in lysogens was demonstrated by its ability to confer rifampin resistance on part of the cellular ribonucleic acid polymerase pool.

Full text

PDF
512

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austin S. J., Tittawella I. P., Hayward R. S., Scaife J. G. Amber mutations of Escherichia coli RNA polymerase. Nat New Biol. 1971 Aug 4;232(31):133–136. doi: 10.1038/newbio232133a0. [DOI] [PubMed] [Google Scholar]
  2. Austin S., Scaife J. A new method for selecting RNA polymerase mutants. J Mol Biol. 1970 Apr 14;49(1):263–267. doi: 10.1016/0022-2836(70)90394-3. [DOI] [PubMed] [Google Scholar]
  3. Burgess R. R. Separation and characterization of the subunits of ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6168–6176. [PubMed] [Google Scholar]
  4. Buxton R. S. Genetic analysis of Escherichia coli K12 mutants resistant to bacteriophage BF23 and the E-group colicins. Mol Gen Genet. 1971;113(2):154–156. doi: 10.1007/BF00333188. [DOI] [PubMed] [Google Scholar]
  5. Carbon J., Squires C., Hill C. W. Glycine transfer RNA of Escherichia coli. II. Impaired GGA-recognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation. J Mol Biol. 1970 Sep 28;52(3):571–584. doi: 10.1016/0022-2836(70)90420-1. [DOI] [PubMed] [Google Scholar]
  6. Gottesman S., Beckwith J. R. Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene. J Mol Biol. 1969 Aug 28;44(1):117–127. doi: 10.1016/0022-2836(69)90408-2. [DOI] [PubMed] [Google Scholar]
  7. Heil A., Zillig W. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription. FEBS Lett. 1970 Dec;11(3):165–168. doi: 10.1016/0014-5793(70)80519-1. [DOI] [PubMed] [Google Scholar]
  8. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ippen K., Shapiro J. A., Beckwith J. R. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages. J Bacteriol. 1971 Oct;108(1):5–9. doi: 10.1128/jb.108.1.5-9.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jasper P., Whitney E., Silver S. Genetic locus determining resistance to phage BF23 and colicins E 1 , E 2 and E 3 in Escherichia coli. Genet Res. 1972 Jun;19(3):305–312. doi: 10.1017/s0016672300014555. [DOI] [PubMed] [Google Scholar]
  11. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  12. Orias E., Gartner T. K., Lannan J. E., Betlach M. Close linkage between ochre and missense suppressors in Escherichia coli. J Bacteriol. 1972 Mar;109(3):1125–1133. doi: 10.1128/jb.109.3.1125-1133.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  14. Signer E. R. Interaction of prophages at the att80 site with the chromosome of Escherichia coli. J Mol Biol. 1966 Jan;15(1):243–255. doi: 10.1016/s0022-2836(66)80224-3. [DOI] [PubMed] [Google Scholar]
  15. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES