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. 1973 Nov;116(2):511–516. doi: 10.1128/jb.116.2.511-516.1973

Isolation and Characterization of φ80 Transducing Bacteriophage for a Ribonucleic Acid Polymerase Gene

Bruce Konrad a,1, Joel Kirschbaum a, Stuart Austin a
PMCID: PMC285411  PMID: 4583236

Abstract

A new method for isolating specialized transducing phages is described. It was used to isolate a group of φ80 transducing phages which carry various bacterial markers from the metB region of the Escherichia coli chromosome. Some of the phages selected for transduction of the supA36 marker were also shown to carry rif, a locus known to specify the β subunit of ribonucleic acid polymerase. Expression of the prophage rifr gene in lysogens was demonstrated by its ability to confer rifampin resistance on part of the cellular ribonucleic acid polymerase pool.

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Selected References

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