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. 1989 Aug;86(15):5933–5937. doi: 10.1073/pnas.86.15.5933

Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone.

G A Bishop 1, J A Frelinger 1
PMCID: PMC297745  PMID: 2788281

Abstract

CH12.LX B cells are responsive to antigen-dependent differentiative signals transmitted through their surface Ek molecules. Although CH12.LX cells express surface Ak molecules with normal protein sequence, the Ak molecules do not deliver differentiative signal to these B cells. To determine whether introduction of a new A molecule into CH12.LX cells would correct this deficiency, CH12.LX cells were transfected with the genes encoding new Aalpha and/or Abeta molecules. It was found that transfected cells responded to antigen-specific signals delivered via the Ealphak Ebetak, Aalphab Abetab, or Aalphak Abetab molecule. However, the B cells did not respond to signals generated by the molecule Aalpha kAbetak, Aalphab Abetak, Aalphak Abetad, or Aalpha kAbetau. Comparison of these sequences suggests that two Abeta residues, His-47 and Trp-197, are important to the transmission of differentiative signals to B cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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