Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1989 Aug 25;17(16):6713–6719. doi: 10.1093/nar/17.16.6713

A physical map of the genome of Ureaplasma urealyticum 960T with ribosomal RNA loci.

B G Cocks 1, L E Pyle 1, L R Finch 1
PMCID: PMC318361  PMID: 2780294

Abstract

A physical map is presented for the 900 kilobase pair genome of Ureaplasma urealyticum 960T, locating 29 sites for 6 restriction endonucleases. The large restriction fragments were separated and sized by pulsed-field agarose gel electrophoresis (PFGE). Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double digestions and partial digestions. An end-labelling technique was used to detect small fragments not readily observed by PFGE. Two loci for rRNA genes have been determined by probing with cloned DNA.

Full text

PDF
6718

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amikam D., Razin S., Glaser G. Ribosomal RNA genes in Mycoplasma. Nucleic Acids Res. 1982 Jul 24;10(14):4215–4222. doi: 10.1093/nar/10.14.4215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bautsch W. Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques. Nucleic Acids Res. 1988 Dec 23;16(24):11461–11467. doi: 10.1093/nar/16.24.11461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Pyle L. E., Corcoran L. N., Cocks B. G., Bergemann A. D., Whitley J. C., Finch L. R. Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes. Nucleic Acids Res. 1988 Jul 11;16(13):6015–6025. doi: 10.1093/nar/16.13.6015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Pyle L. E., Finch L. R. A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci. Nucleic Acids Res. 1988 Jul 11;16(13):6027–6039. doi: 10.1093/nar/16.13.6027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Razin S., Barile M. F., Harasawa R., Amikam D., Glaser G. Characterization of the mycoplasma genome. Yale J Biol Med. 1983 Sep-Dec;56(5-6):357–366. [PMC free article] [PubMed] [Google Scholar]
  6. Romano N., Tolone G., Ajello F., La Licata R. Adenosine 5'-triphosphate synthesis induced by urea hydrolysis in Ureaplasma urealyticum. J Bacteriol. 1980 Nov;144(2):830–832. doi: 10.1128/jb.144.2.830-832.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Samuelsson T., Elias P., Lustig F., Guindy Y. S. Cloning and nucleotide sequence analysis of transfer RNA genes from Mycoplasma mycoides. Biochem J. 1985 Nov 15;232(1):223–228. doi: 10.1042/bj2320223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Smith C. L., Econome J. G., Schutt A., Klco S., Cantor C. R. A physical map of the Escherichia coli K12 genome. Science. 1987 Jun 12;236(4807):1448–1453. doi: 10.1126/science.3296194. [DOI] [PubMed] [Google Scholar]
  9. Wenzel R., Herrmann R. Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res. 1988 Sep 12;16(17):8323–8336. doi: 10.1093/nar/16.17.8323. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES