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. 1989 Aug 25;17(16):6713–6719. doi: 10.1093/nar/17.16.6713

A physical map of the genome of Ureaplasma urealyticum 960T with ribosomal RNA loci.

B G Cocks 1, L E Pyle 1, L R Finch 1
PMCID: PMC318361  PMID: 2780294

Abstract

A physical map is presented for the 900 kilobase pair genome of Ureaplasma urealyticum 960T, locating 29 sites for 6 restriction endonucleases. The large restriction fragments were separated and sized by pulsed-field agarose gel electrophoresis (PFGE). Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double digestions and partial digestions. An end-labelling technique was used to detect small fragments not readily observed by PFGE. Two loci for rRNA genes have been determined by probing with cloned DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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