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. 1989 Sep 12;17(17):7029–7043. doi: 10.1093/nar/17.17.7029

Cloning of the complete Mycoplasma pneumoniae genome.

R Wenzel 1, R Herrmann 1
PMCID: PMC318431  PMID: 2506532

Abstract

The complete genome of Mycoplasma pneumoniae was cloned in an ordered library consisting of 34 overlapping or adjacent cosmids, one plasmid and two lambda phages. The genome size was determined by adding up the sizes of either the individual unique EcoRI restriction fragments of the gene bank or of the XhoI fragments of genomic M. pneumoniae DNA. The values from these calculations, 835 and 849 kbp, are in good agreement. An XhoI restriction map was constructed by identifying adjacent DNA fragments by probing with selected cosmid clones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bak A. L., Black F. T., Christiansen C., Freundt E. A. Genome size of mycoplasmal DNA. Nature. 1969 Dec 20;224(5225):1209–1210. doi: 10.1038/2241209a0. [DOI] [PubMed] [Google Scholar]
  2. Bernardi A., Bernardi F. Complete sequence of pSC101. Nucleic Acids Res. 1984 Dec 21;12(24):9415–9426. doi: 10.1093/nar/12.24.9415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bode H. R., Morowitz H. J. Size and structure of the Mycoplasma hominis H39 chromosome. J Mol Biol. 1967 Jan 28;23(2):191–199. doi: 10.1016/s0022-2836(67)80026-3. [DOI] [PubMed] [Google Scholar]
  4. Carle G. F., Frank M., Olson M. V. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science. 1986 Apr 4;232(4746):65–68. doi: 10.1126/science.3952500. [DOI] [PubMed] [Google Scholar]
  5. Clarke L., Carbon J. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 1979;68:396–408. doi: 10.1016/0076-6879(79)68029-1. [DOI] [PubMed] [Google Scholar]
  6. Darai G., Zöller L., Matz B., Delius H., Speck P. T., Flügel R. M. Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. J Bacteriol. 1982 May;150(2):788–794. doi: 10.1128/jb.150.2.788-794.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  8. Loechel S., Inamine J. M., Hu P. C. Nucleotide sequence of the deoC gene of Mycoplasma pneumoniae. Nucleic Acids Res. 1989 Jan 25;17(2):801–801. doi: 10.1093/nar/17.2.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maniloff J. Anomalous values of Mycoplasma genomes sizes determined by pulse-field gel electrophoresis. Nucleic Acids Res. 1989 Feb 11;17(3):1268–1268. doi: 10.1093/nar/17.3.1268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McClelland M., Jones R., Patel Y., Nelson M. Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res. 1987 Aug 11;15(15):5985–6005. doi: 10.1093/nar/15.15.5985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morowitz H. J. The completeness of molecular biology. Isr J Med Sci. 1984 Sep;20(9):750–753. [PubMed] [Google Scholar]
  12. Nassal M., Mogi T., Karnik S. S., Khorana H. G. Structure-function studies on bacteriorhodopsin. III. Total synthesis of a gene for bacterio-opsin and its expression in Escherichia coli. J Biol Chem. 1987 Jul 5;262(19):9264–9270. [PubMed] [Google Scholar]
  13. Poddar S. K., Maniloff J. Determination of microbial genome sizes by two-dimensional denaturing gradient gel electrophoresis. Nucleic Acids Res. 1989 Apr 25;17(8):2889–2895. doi: 10.1093/nar/17.8.2889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pyle L. E., Corcoran L. N., Cocks B. G., Bergemann A. D., Whitley J. C., Finch L. R. Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes. Nucleic Acids Res. 1988 Jul 11;16(13):6015–6025. doi: 10.1093/nar/16.13.6015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  16. Short J. M., Fernandez J. M., Sorge J. A., Huse W. D. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res. 1988 Aug 11;16(15):7583–7600. doi: 10.1093/nar/16.15.7583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Su C. J., Chavoya A., Baseman J. B. Regions of Mycoplasma pneumoniae cytadhesin P1 structural gene exist as multiple copies. Infect Immun. 1988 Dec;56(12):3157–3161. doi: 10.1128/iai.56.12.3157-3161.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wenzel R., Herrmann R. Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res. 1988 Sep 12;16(17):8323–8336. doi: 10.1093/nar/16.17.8323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wenzel R., Herrmann R. Repetitive DNA sequences in Mycoplasma pneumoniae. Nucleic Acids Res. 1988 Sep 12;16(17):8337–8350. doi: 10.1093/nar/16.17.8337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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