Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Feb 24;12(4):2181–2192. doi: 10.1093/nar/12.4.2181

The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.

J M Zengel, R H Archer, L Lindahl
PMCID: PMC318650  PMID: 6322136

Abstract

We have determined the nucleotide sequence of the Escherichia coli fus gene, which codes for elongation factor G. The protein product of the sequenced gene contains 703 amino acids, with a predicted molecular weight of 77,444. The fus gene shows the nonrandom pattern of codon usage typical of ribosomal proteins and other proteins synthesized at a high level. We have identified several potential promoter sequences within the gene. One of these sequences may correspond to the secondary promoter for expression of the downstream tufA gene (encoding elongation factor Tu) whose activity has been described previously (1,2). A comparison of the nucleotide and amino acid sequences of elongation factors G and Tu reveals a limited but significant homology between the two proteins within the 150 amino acid residues at their amino-terminal ends.

Full text

PDF
2183

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. An G., Friesen J. D. The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. Gene. 1980 Dec;12(1-2):33–39. doi: 10.1016/0378-1119(80)90013-x. [DOI] [PubMed] [Google Scholar]
  2. An G., Lee J. S., Friesen J. D. Evidence for an internal promoter preceding tufA in the str operon of Escherichia coli. J Bacteriol. 1982 Feb;149(2):548–553. doi: 10.1128/jb.149.2.548-553.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cerretti D. P., Dean D., Davis G. R., Bedwell D. M., Nomura M. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 1983 May 11;11(9):2599–2616. doi: 10.1093/nar/11.9.2599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ikemura T. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system. J Mol Biol. 1981 Sep 25;151(3):389–409. doi: 10.1016/0022-2836(81)90003-6. [DOI] [PubMed] [Google Scholar]
  6. Jaskunas S. R., Lindahl L., Nomura M. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Nature. 1975 Oct 9;257(5526):458–462. doi: 10.1038/257458a0. [DOI] [PubMed] [Google Scholar]
  7. Jaskunas S. R., Nomura M. Organization of ribosomal protein genes of Escherichia coli as analyzed by polar insertion mutations. J Biol Chem. 1977 Oct 25;252(20):7337–7343. [PubMed] [Google Scholar]
  8. Jones M. D., Petersen T. E., Nielsen K. M., Magnusson S., Sottrup-Jensen L., Gausing K., Clark B. F. The complete amino-acid sequence of elongation factor Tu from Escherichia coli. Eur J Biochem. 1980 Jul;108(2):507–526. doi: 10.1111/j.1432-1033.1980.tb04748.x. [DOI] [PubMed] [Google Scholar]
  9. Laursen R. A., Duffy L. The evolution of elongation factors Tu and G by gene duplication. FEBS Lett. 1978 Aug 15;92(2):200–202. doi: 10.1016/0014-5793(78)80753-4. [DOI] [PubMed] [Google Scholar]
  10. Lindahl L., Zengel J. M. Expression of ribosomal genes in bacteria. Adv Genet. 1982;21:53–121. doi: 10.1016/s0065-2660(08)60297-7. [DOI] [PubMed] [Google Scholar]
  11. Ovchinnikov YuA, Alakhov YuB, Bundulis YuP, Bundule M. A., Dovgas N. V., Kozlov V. P., Motuz L. P., Vinokurov L. M. The primary structure of elongation factor G from Escherichia coli. A complete amino acid sequence. FEBS Lett. 1982 Mar 8;139(1):130–135. doi: 10.1016/0014-5793(82)80503-6. [DOI] [PubMed] [Google Scholar]
  12. Polisky B., Bishop R. J., Gelfand D. H. A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3900–3904. doi: 10.1073/pnas.73.11.3900. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Post L. E., Nomura M. DNA sequences from the str operon of Escherichia coli. J Biol Chem. 1980 May 25;255(10):4660–4666. [PubMed] [Google Scholar]
  14. Post L. E., Strycharz G. D., Nomura M., Lewis H., Dennis P. P. Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1697–1701. doi: 10.1073/pnas.76.4.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pustell J., Kafatos F. C. A convenient and adaptable package of DNA sequence analysis programs for microcomputers. Nucleic Acids Res. 1982 Jan 11;10(1):51–59. doi: 10.1093/nar/10.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pustell J., Kafatos F. C. A high speed, high capacity homology matrix: zooming through SV40 and polyoma. Nucleic Acids Res. 1982 Aug 11;10(15):4765–4782. doi: 10.1093/nar/10.15.4765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yokota T., Sugisaki H., Takanami M., Kaziro Y. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Gene. 1980 Dec;12(1-2):25–31. doi: 10.1016/0378-1119(80)90012-8. [DOI] [PubMed] [Google Scholar]
  20. Zengel J. M., Lindahl L. A secondary promoter for elongation factor Tu synthesis in the str ribosomal protein operon of Escherichia coli. Mol Gen Genet. 1982;185(3):487–492. doi: 10.1007/BF00334145. [DOI] [PubMed] [Google Scholar]
  21. Zengel J. M., Lindahl L. Oversynthesis of elongation factors G and Tu in Escherichia coli. J Bacteriol. 1982 Feb;149(2):793–797. doi: 10.1128/jb.149.2.793-797.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES