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. 1984 Jun 11;12(11):4481–4492. doi: 10.1093/nar/12.11.4481

Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence.

M R Fung, R M Campbell, R T MacGillivray
PMCID: PMC318852  PMID: 6330671

Abstract

Thirty thousand colonies of a bovine liver cDNA library were screened with a mixture of synthetic oligodeoxyribonucleotides coding for bovine factor X. Five positive colonies were identified, and plasmid DNA was isolated. Cleavage with restriction endonucleases showed that these plasmids (designated pBX1 -5) contained inserts of 1530bp , 770bp , 700bp , 1100bp and 930bp . DNA sequence analysis of the plasmid with the largest insert ( pBX1 ) confirmed that bovine factor X cDNAs had been cloned. The cDNA sequence predicts that factor X is synthesized as a single chain precursor in which the light and heavy chains of plasma factor X are linked by the dipeptide Arg-Arg. The cDNA sequence also predicts that factor X is synthesized with a prepro leader peptide. We propose that at least five specific proteolytic events occur during the conversion of prepro -factor X to plasma factor Xa.

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Selected References

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