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. 1984 Jun 25;12(12):4977–4985. doi: 10.1093/nar/12.12.4977

The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene.

J Swindle, J Ajioka, D Dawson, R Myers, D Carroll, C Georgopoulos
PMCID: PMC318892  PMID: 6330693

Abstract

The nusB (groNB) gene product of Escherichia coli plays a pivotal role in allowing bacteriophage lambda N protein to function as an antiterminator of mRNA transcription and in modulating host gene expression. In addition it is essential for bacterial viability since mutations in it result in a cold-sensitivity phenotype for growth. We have previously cloned the nusB gene and shown it to code for a 14,500-Mr protein. Here we present the primary DNA sequence of the nusB gene. From the sequence we deduce that it codes for a slightly basic protein (21 basic as opposed to 20 acidic amino acids) composed of 139 amino acids with a cumulative 15,689-Mr. The predicted N-terminal amino acid sequence as well as the overall amino acid composition agrees well with that of the purified protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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