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. 1984 Aug 10;12(15):6291–6305. doi: 10.1093/nar/12.15.6291

The B----Z transition in two synthetic oligonucleotides: d(C-2-amino-ACGTG) and d(m5CGCAm5CGTGCG) studied by IR, NMR and CD spectroscopies.

J A Taboury, S Adam, E Taillandier, J M Neumann, S Tran-Dinh, T Huynh-Dinh, B Langlois d'Estaintot, M Conti, J Igolen
PMCID: PMC320073  PMID: 6332307

Abstract

The sequences CA'CGTG (where A' = 2-aminodeoxyadenosine) and m5CGCAm5CGTGCG are prepared and studied by IR, CD and 1H-NMR. Infrared spectra demonstrate the capacity of the modified hexamer and decamer to adopt a Z conformation. The influence of the NH2 substitution on the adenine or of the methylated terminal part of the decamer acting with the increase of the DNA concentration stabilizes the Z conformation at room temperature in low humidity films. Very weak proportion of Z conformation is detected in UV dilute solutions. In more concentrated NMR solutions, the Z proportion induced by high salt content is only 20-25%. The effects of the concentration and of the covalent modification of the bases are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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