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. 1985 Nov 11;13(21):7639–7645. doi: 10.1093/nar/13.21.7639

Reduced cleavage by sodium hydroxide of methyladenine in DNA sequencing.

N Okawa, Y Suyama, A Kaji
PMCID: PMC322076  PMID: 4069995

Abstract

When the Maxam and Gilbert DNA sequencing method which is modified by Bencini et al. (Biotechniques Jan/Feb pp4-5, 1984) is applied to DNA containing methylated adenine in a GATC sequence, the cleavage reaction by sodium hydroxide is found to be greatly reduced in comparison to that of non-methylated adenine. Thus, a faint band in A greater than C reaction suggests a methyl adenine and can be used for its detection. That the faint band corresponds to a methyladenine was confirmed by Sanger sequencing of the same fragment and further by Maxam and Gilbert sequencing of the complementary strand of DNA, which was replicated in an E. coli strain either having or lacking methylation enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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