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. 1970 Apr;49(4):806–812. doi: 10.1172/JCI106294

Effect of 2,3-diphosphoglycerate on oxygen affinity of blood in sickle cell anemia

Samuel Charache 1,2,3, Santiago Grisolia 1,2,3, Adam J Fiedler 1,2,3, Andre E Hellegers 1,2,3
PMCID: PMC322537  PMID: 5443181

Abstract

Blood of patients with sickle cell anemia (SS) exhibits decreased affinity for oxygen, although the oxygen affinity of hemoglobin S is the same as that of hemoglobin A. SS red cells contain more 2,3-diphosphoglycerate (DPG) than normal erythrocytes. The oxygen affinity of hemolyzed red cells is decreased by added DPG, and hemolysates prepared from SS red cells do not differ from normal hemolysates in this regard. Reduction of oxygen affinity to the levels found in intact SS red cells required DPG concentrations in excess of those found in most SS patients. The same was true of oxygen affinity of patients with pyruvate kinase deficiency. Other organic phosphates, as well as inorganic ions, are known to alter the oxygen affinity of dilute solutions of hemoglobin. These substances, the state of aggregation of hemoglobin molecules, and cytoarchitectural factors probably play roles in determining oxygen affinity of both normal and SS red cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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