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. 1986 Apr;83(8):2448–2452. doi: 10.1073/pnas.83.8.2448

Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factor.

M Klagsbrun, J Sasse, R Sullivan, J A Smith
PMCID: PMC323315  PMID: 3458207

Abstract

A human hepatoma cell line synthesizes, as evidenced by metabolic labeling, an endothelial cell mitogen that is found to be mostly cell associated. The hepatoma-derived growth factor (HDGF) has been purified to homogeneity by a combination of Bio-Rex 70, heparin-Sepharose, and reverse-phase chromatography; it is a cationic polypeptide with a molecular weight of about 18,500-19,000. HDGF is structurally related to basic fibroblast growth factor (FGF). Immunological analysis demonstrates that antiserum prepared against a synthetic peptide corresponding to the amino-terminal sequence of basic FGF cross-reacts with HDGF when analyzed by electrophoretic blotting and by immunoprecipitation. Sequence analysis of tryptic fragments demonstrates that HDGF contains sequences that are homologous to both amino-terminal and carboxyl-terminal sequences of basic FGF.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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