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. 1980 Jun 11;8(11):2377–2395. doi: 10.1093/nar/8.11.2377

An experimentally-derived model for the secondary structure of the 16S ribosomal RNA from Escherichia coli.

C Glotz, R Brimacombe
PMCID: PMC324088  PMID: 6160459

Abstract

Ribonucleoprotein fragments are isolated by mild ribonuclease digestion of E. coli 30S ribosomal subunits, and are deproteinized and subjected to a second partial digestion. Base-pairing between the resulting small RNA fragments is investigated using the two-dimensional gel electrophoresis procedure already reported (see Ref. 1). The interactions thus found are incorporated into a secondary structure model covering approximately 80% of the 16S RNA.

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Selected References

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