Abstract
ScV-L is a simple double-stranded RNA virus of yeast, consisting of a 4.8 kilobase pair double-stranded RNA (L) encapsidated in isometric particles composed mainly of one polypeptide (ScV-Pl) of 88,000 daltons. L encodes ScV-Pl. There is a capsid-associated RNA polymerase that synthesizes in vitro predominantly single-stranded RNA. We show that this polymerase activity is a transcriptase, at the least one product of which is the mRNA for ScV-Pl. The transcript, like its template, is uncapped.
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- Bevan E. A., Herring A. J., Mitchell D. J. Preliminary characterization of two species of dsRNA in yeast and their relationship to the "killer" character. Nature. 1973 Sep 14;245(5420):81–86. doi: 10.1038/245081b0. [DOI] [PubMed] [Google Scholar]
- Bostian K. A., Hopper J. E., Rogers D. T., Tipper D. J. Translational analysis of the killer-associated virus-like particle dsRNA genome of S. cerevisiae: M dsRNA encodes toxin. Cell. 1980 Feb;19(2):403–414. doi: 10.1016/0092-8674(80)90514-0. [DOI] [PubMed] [Google Scholar]
- Bruenn J. A., Brennan V. E. Yeast viral double-stranded RNAs have heterogeneous 3' termini. Cell. 1980 Apr;19(4):923–933. doi: 10.1016/0092-8674(80)90084-7. [DOI] [PubMed] [Google Scholar]
- Bruenn J., Kane W. Relatedness of the double-stranded RNAs present in yeast virus-like particles. J Virol. 1978 Jun;26(3):762–772. doi: 10.1128/jvi.26.3.762-772.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bruenn J., Keitz B. The 5' ends of yeast killer factor RNAs are pppGp. Nucleic Acids Res. 1976 Oct;3(10):2427–2436. doi: 10.1093/nar/3.10.2427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buck K. W. Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S. Nucleic Acids Res. 1975 Oct;2(10):1889–1902. doi: 10.1093/nar/2.10.1889. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chater K. F., Morgan D. H. Ribonucleic acid synthesis by isolated viruses of Penicillium stoloniferum. J Gen Virol. 1974 Aug;24(2):307–317. doi: 10.1099/0022-1317-24-2-307. [DOI] [PubMed] [Google Scholar]
- Efstratiadis A., Vournakis J. N., Donis-Keller H., Chaconas G., Dougall D. K., Kafatos F. C. End labeling of enzymatically decapped mRNA. Nucleic Acids Res. 1977 Dec;4(12):4165–4174. doi: 10.1093/nar/4.12.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fried H. M., Fink G. R. Electron microscopic heteroduplex analysis of "killer" double-stranded RNA species from yeast. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4224–4228. doi: 10.1073/pnas.75.9.4224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris M. S. Virus-like particles and double stranded RNA from killer and non-killer strains of Saccharomyces cerevisiae. Microbios. 1978;21(85-86):161–176. [PubMed] [Google Scholar]
- Hastie N. D., Brennan V., Bruenn J. A. No homology between double-stranded RNA and nuclear DNA of yeast. J Virol. 1978 Dec;28(3):1002–1005. doi: 10.1128/jvi.28.3.1002-1005.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Held W. A., West K., Gallagher J. F. Importance of initiation factor preparations in the translation of reovirus and globin mRNAs lacking a 5'-terminal 7-methylguanosine. J Biol Chem. 1977 Dec 10;252(23):8489–8497. [PubMed] [Google Scholar]
- Herring A. J., Bevan E. A. Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. J Gen Virol. 1974 Mar;22(3):387–394. doi: 10.1099/0022-1317-22-3-387. [DOI] [PubMed] [Google Scholar]
- Herring A. J., Bevan E. A. Yeast virus-like particles possess a capsid-associated single-stranded RNA polymerase. Nature. 1977 Aug 4;268(5619):464–466. doi: 10.1038/268464a0. [DOI] [PubMed] [Google Scholar]
- Hollings M. Mycoviruses: viruses that infect fungi. Adv Virus Res. 1978;22:1–53. doi: 10.1016/s0065-3527(08)60771-x. [DOI] [PubMed] [Google Scholar]
- Holm C. A., Oliver S. G., Newman A. M., Holland L. E., McLaughlin C. S., Wagner E. K., Warner R. C. The molecular weight of yeast P1 double-stranded RNA. J Biol Chem. 1978 Nov 25;253(22):8332–8336. [PubMed] [Google Scholar]
- Hopper J. E., Bostian K. A., Rowe L. B., Tipper D. J. Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae. J Biol Chem. 1977 Dec 25;252(24):9010–9017. [PubMed] [Google Scholar]
- Kane W. P., Pietras D. F., Bruenn J. A. Evolution of defective-interfering double-stranded RNAs of the yeast killer virus. J Virol. 1979 Nov;32(2):692–696. doi: 10.1128/jvi.32.2.692-696.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Nash C. H., Douthart R. J., Ellis L. F., Van Frank R. M., Burnett J. P., Lemke P. A. On the mycophage of Penicillium chrysogenum. Can J Microbiol. 1973 Jan;19(1):97–103. doi: 10.1139/m73-014. [DOI] [PubMed] [Google Scholar]
- Palfree R. G., Bussey H. Yeast killer toxin: purification and characterisation of the protein toxin from Saccharomyces cerevisiae. Eur J Biochem. 1979 Feb 1;93(3):487–493. doi: 10.1111/j.1432-1033.1979.tb12847.x. [DOI] [PubMed] [Google Scholar]
- Ratti G., Buck K. W. RNA polymerase activity in double-stranded ribonucleic acid virus particles from Aspergillus foetidus. Biochem Biophys Res Commun. 1975 Sep 16;66(2):706–711. doi: 10.1016/0006-291x(75)90567-7. [DOI] [PubMed] [Google Scholar]
- Ratti G., Buck K. W. Semi-conservative transcription in particles of a double-stranded RNA mycovirus. Nucleic Acids Res. 1978 Oct;5(10):3843–3854. doi: 10.1093/nar/5.10.3843. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schreier M. H., Erni B., Staehelin T. Initiation of mammalian protein synthesis. I. Purification and characterization of seven initiation factors. J Mol Biol. 1977 Nov;116(4):727–753. doi: 10.1016/0022-2836(77)90268-6. [DOI] [PubMed] [Google Scholar]
- Schreier M. H., Staehelin T. Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systems. J Mol Biol. 1973 Feb 19;73(3):329–349. doi: 10.1016/0022-2836(73)90346-x. [DOI] [PubMed] [Google Scholar]
- Silverstein S. C., Christman J. K., Acs G. The reovirus replicative cycle. Annu Rev Biochem. 1976;45:375–408. doi: 10.1146/annurev.bi.45.070176.002111. [DOI] [PubMed] [Google Scholar]
- Somers J. M. Isolation of Suppressive Sensitive Mutants from Killer and Neutral Strains of SACCHAROMYCES CEREVISIAE. Genetics. 1973 Aug;74(4):571–579. doi: 10.1093/genetics/74.4.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Etten J. L., Burbank D. E., Cuppels D. A., Lane L. C., Vidaver A. K. Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase. J Virol. 1980 Feb;33(2):769–773. doi: 10.1128/jvi.33.2.769-773.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wickner R. B. The killer double-stranded RNA plasmids of yeast. Plasmid. 1979 Jul;2(3):303–322. doi: 10.1016/0147-619x(79)90015-5. [DOI] [PubMed] [Google Scholar]
- Wickner R. B. Twenty-six chromosomal genes needed to maintain the killer double-stranded RNA plasmid of Saccharomyces cerevisiae. Genetics. 1978 Mar;88(3):419–425. doi: 10.1093/genetics/88.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]