Abstract
The structure of a modified uridine derivative which was detected at the first letter position of the anticodon of Bacillus subtilis tRNA1Lys was determined to be 5-(carboxymethylaminomethyl)-2-thiouridine. The determination was mainly based in this ultraviolet absorption spectra and mass spectrometric analysis of the trimethylsilyl derivative.
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- Carbon J., David H., Studier M. H. Thiobases in Escherchia coli Transfer RNA: 2-Thiocytosine and 5-Methylaminomethyl-2-thiouracil. Science. 1968 Sep 13;161(3846):1146–1147. doi: 10.1126/science.161.3846.1146. [DOI] [PubMed] [Google Scholar]
- Chakraburtty K., Steinschneider A., Case R. V., Mehler A. H. Primary structure of tRNA-Lys of E. coli B. Nucleic Acids Res. 1975 Nov;2(11):2069–2075. doi: 10.1093/nar/2.11.2069. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murao K., Hasegawa T., Ishikura H. 5-methoxyuridine: a new minor constituent located in the first position of the anticodon of tRNAAla, tRNAThr, and tRNAVal from Bacillus subtilis. Nucleic Acids Res. 1976 Oct;3(10):2851–2860. doi: 10.1093/nar/3.10.2851. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
- Saneyoshi M., Nishimura S. Selective modification of 4-thiouridylate residue in Escherichia coli transfer RNA with cyanogen bromide. Biochim Biophys Acta. 1970 Apr 15;204(2):389–399. doi: 10.1016/0005-2787(70)90158-9. [DOI] [PubMed] [Google Scholar]
- Sen G. C., Ghosh H. P. Role of modified nucleosides in tRNA: effect of modification of the 2-thiouridine derivative located at the 5'-end of the anticodon of yeast transfer RNA Lys2. Nucleic Acids Res. 1976 Mar;3(3):523–535. doi: 10.1093/nar/3.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamada Y., Ishikura H. Nucleotide sequence of a lysine tRNA from Bacillus subtilis. Nucleic Acids Res. 1977 Dec;4(12):4291–4303. doi: 10.1093/nar/4.12.4291. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoshida M., Takeishi K., Ukita T. Structural studies on a yeast glutamic acid tRNA specific to GAA codon. Biochim Biophys Acta. 1971 Jan 1;228(1):153–166. doi: 10.1016/0005-2787(71)90555-7. [DOI] [PubMed] [Google Scholar]