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. 1991 Jun 11;19(11):2835–2839. doi: 10.1093/nar/19.11.2835

31P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/KHSO5' in comparison with DNases I and II.

G Gasmi 1, M Pasdeloup 1, G Pratviel 1, M Pitié 1, J Bernadou 1, B Meunier 1
PMCID: PMC328240  PMID: 2057347

Abstract

Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.

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Selected References

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