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. 1992 Jun 11;20(11):2705–2710. doi: 10.1093/nar/20.11.2705

Structural and functional features of cis-acting sequences in the basic replicon of plasmid ColIb-P9.

K Tanaka 1, H Sakai 1, Y Honda 1, T Nakamura 1, A Higashi 1, T Komano 1
PMCID: PMC336911  PMID: 1614857

Abstract

We have structurally and functionally analyzed the cis-elements essential for ColIb-P9 plasmid DNA replication. The putative oriV region encompassed a region of 172 base pairs (bp) located 152 bp downstream of the repZ gene. A typical dnaA box found in this region proved nonessential for the DNA replication of ColIb-P9. The ssi signal of ColIb-P9 is a homologue of the G-sites of R1 and R100 plasmids. Deletion of the G-site led to 1.5-fold reduction of the copy number, suggesting that although this G-site is not essential, it is important for efficient ColIb-P9 DNA replication. In addition, the ColIb-P9 replicon is highly and extensively homologous with the P307 (RepFIC) replicon, and highly homologous with the R100 (RepFIIA) replicon around the G-site region. These facts imply a common ancestry from which the plasmids have evolved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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