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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jan;81(1):145–149. doi: 10.1073/pnas.81.1.145

Calculation of the polarized fluorescence from a labeled muscle fiber.

M F Morales
PMCID: PMC344627  PMID: 6582471

Abstract

Equations are derived that explicitly relate fluorescence polarization observables on a labeled muscle fiber to attitude of the cross-bridges and to attitude of the labels within the cross-bridges.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson J. F., Morales M. F. Polarization of tryptophan fluorescence in muscle. Biochemistry. 1969 Nov;8(11):4517–4522. doi: 10.1021/bi00839a044. [DOI] [PubMed] [Google Scholar]
  2. Borejdo J., Assulin O., Ando T., Putnam S. Cross-bridge orientation in skeletal muscle measured by linear dichroism of an extrinsic chromophore. J Mol Biol. 1982 Jul 5;158(3):391–414. doi: 10.1016/0022-2836(82)90205-4. [DOI] [PubMed] [Google Scholar]
  3. Burghardt T. P., Ando T., Borejdo J. Evidence for cross-bridge order in contraction of glycerinated skeletal muscle. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7515–7519. doi: 10.1073/pnas.80.24.7515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mendelson R. A., Wilson M. G. Three-dimensional disorder of dipolar probes in a helical array. Application to muscle cross-bridges. Biophys J. 1982 Aug;39(2):221–227. doi: 10.1016/S0006-3495(82)84511-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nihei T., Mendelson R. A., Botts J. Use of fluorescence polarization to observe changes in attitude of S-1 moieties in muscle fibers. Biophys J. 1974 Mar;14(3):236–242. doi: 10.1016/S0006-3495(74)85911-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Tregear R. T., Mendelson R. A. Polarization from a helix of fluorophores and its relation to that obtained from muscle. Biophys J. 2009 Jan 1;15(5):455–467. doi: 10.1016/S0006-3495(75)85830-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Winkelmann D. A., Lowey S., Press J. L. Monoclonal antibodies localize changes on myosin heavy chain isozymes during avian myogenesis. Cell. 1983 Aug;34(1):295–306. doi: 10.1016/0092-8674(83)90160-5. [DOI] [PubMed] [Google Scholar]

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