Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Mar;79(5):1639–1642. doi: 10.1073/pnas.79.5.1639

Lipoprotein lipase secretion by human monocytes and rabbit alveolar macrophages in culture.

E M Mahoney, J C Khoo, D Steinberg
PMCID: PMC346031  PMID: 6951202

Abstract

Human peripheral blood monocytes and rabbit alveolar macrophages secreted lipoprotein lipase during culture. Within hours after plating, lipoprotein lipase had accumulated in the culture medium of monocytes, and the rate of accumulation increased with time in culture. The initial rate of secretion of lipoprotein lipase by alveolar macrophages was higher than in monocytes but decreased after 8--10 hr to values similar to those expressed by monocytes cultured for the same length of time. The enzyme was characterized as lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34) on the basis of pH optimum (7.8--8.2 for monocytes, 8.1 for alveolar macrophages), dependence on apolipoprotein C-II for activity, inhibition by 0.3--0.5 M sodium chloride and protamine sulfate, and retention of a heparin-Sepharose gel. The expression of lipoprotein lipase secretion by human monocytes may have important implications with respect to the development of foam cells in the arterial wall during atherogenesis.

Full text

PDF
1641

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bierman E. L., Eisenberg S., Stein O., Stein Y. Very low density lipoprotein "remnant" particles: uptake by aortic smooth muscle cells in culture. Biochim Biophys Acta. 1973 Nov 2;329(1):163–169. doi: 10.1016/0304-4165(73)90021-4. [DOI] [PubMed] [Google Scholar]
  2. Boyum A. Separation of lymphocytes, lymphocyte subgroups and monocytes: a review. Lymphology. 1977 Jun;10(2):71–76. [PubMed] [Google Scholar]
  3. Brown W. V., Levy R. I., Fredrickson D. S. Further separation of the apoproteins of the human plasma very low density lipoproteins. Biochim Biophys Acta. 1970 Mar 31;200(3):573–575. doi: 10.1016/0005-2795(70)90115-7. [DOI] [PubMed] [Google Scholar]
  4. Catapano A. L., Gianturco S. H., Kinnunen P. K., Eisenberg S., Gotto A. M., Jr, Smith L. C. Suppression of 3-hydroxy-3-methylglutaryl-CoA reductase by low density lipoproteins produced in vitro by lipoprotein lipase action on nonsuppressive very low density lipoproteins. J Biol Chem. 1979 Feb 25;254(4):1007–1009. [PubMed] [Google Scholar]
  5. DAY A. J. THE MACROPHAGE SYSTEM, LIPID METABOLISM AND ATHEROSCLEROSIS. J Atheroscler Res. 1964 Mar-Apr;4:117–130. doi: 10.1016/s0368-1319(64)80032-6. [DOI] [PubMed] [Google Scholar]
  6. Florén C. H., Albers J. J., Bierman E. L. Uptake of chylomicron remnants causes cholesterol accumulation in cultured human arterial smooth muscle cells. Biochim Biophys Acta. 1981 Jan 26;663(1):336–349. doi: 10.1016/0005-2760(81)90219-8. [DOI] [PubMed] [Google Scholar]
  7. Fowler S., Shio H., Haley N. J. Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. IV. Investigation of macrophage-like properties of aortic cell populations. Lab Invest. 1979 Oct;41(4):372–378. [PubMed] [Google Scholar]
  8. Gerrity R. G. The role of the monocyte in atherogenesis: I. Transition of blood-borne monocytes into foam cells in fatty lesions. Am J Pathol. 1981 May;103(2):181–190. [PMC free article] [PubMed] [Google Scholar]
  9. Goldstein J. L., Ho Y. K., Basu S. K., Brown M. S. Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc Natl Acad Sci U S A. 1979 Jan;76(1):333–337. doi: 10.1073/pnas.76.1.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goldstein J. L., Ho Y. K., Brown M. S., Innerarity T. L., Mahley R. W. Cholesteryl ester accumulation in macrophages resulting from receptor-mediated uptake and degradation of hypercholesterolemic canine beta-very low density lipoproteins. J Biol Chem. 1980 Mar 10;255(5):1839–1848. [PubMed] [Google Scholar]
  11. Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Green M. R., Lin J. S., Berman L. B., Osman M. M., Cerreta J. M., Mandl I., Turino G. M. Elastolytic activity of alveolar macrophages in normal dogs and human subjects. J Lab Clin Med. 1979 Oct;94(4):549–562. [PubMed] [Google Scholar]
  13. Henriksen T., Mahoney E. M., Steinberg D. Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells: recognition by receptors for acetylated low density lipoproteins. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6499–6503. doi: 10.1073/pnas.78.10.6499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Iverius P. H. Coupling of glycosaminoglycans to agarose beads (sepharose 4B). Biochem J. 1971 Oct;124(4):677–683. doi: 10.1042/bj1240677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Johnson W. D., Jr, Mei B., Cohn Z. A. The separation, long-term cultivation, and maturation of the human monocyte. J Exp Med. 1977 Dec 1;146(6):1613–1626. doi: 10.1084/jem.146.6.1613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KORN E. D., QUIGLEY T. W., Jr Lipoprotein lipase of chicken adipose tissue. J Biol Chem. 1957 Jun;226(2):833–839. [PubMed] [Google Scholar]
  17. Khoo J. C., Mahoney E. M., Witztum J. L. Secretion of lipoprotein lipase by macrophages in culture. J Biol Chem. 1981 Jul 25;256(14):7105–7108. [PubMed] [Google Scholar]
  18. Khoo J. C., Steinberg D., Huang J. J., Vagelos P. R. Triglyceride, diglyceride, monoglyceride, and cholesterol ester hydrolases in chicken adipose tissue activated by adenosine 3':5'-Monophosphate-dependent protein kinase. Chromatographic resolution and immunochemical differentiation from lipoprotein lipase. J Biol Chem. 1976 May 25;251(10):2882–2890. [PubMed] [Google Scholar]
  19. MYRVIK Q., LEAKE E. S., FARISS B. Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961 Feb;86:128–132. [PubMed] [Google Scholar]
  20. Mahley R. W., Innerarity T. L., Weisgraber K. B., Oh S. Y. Altered metabolism (in vivo and in vitro) of plasma lipoproteins after selective chemical modification of lysine residues of the apoproteins. J Clin Invest. 1979 Sep;64(3):743–750. doi: 10.1172/JCI109518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nathan C. F., Murray H. W., Cohn Z. A. The macrophage as an effector cell. N Engl J Med. 1980 Sep 11;303(11):622–626. doi: 10.1056/NEJM198009113031106. [DOI] [PubMed] [Google Scholar]
  22. Pietra G. G., Spagnoli L. G., Capuzzi D. M., Sparks C. E., Fishman A. P., Marsh J. B. Metabolism of 125I-labeled lipoproteins by the isolated rat lung. J Cell Biol. 1976 Jul;70(1):33–46. doi: 10.1083/jcb.70.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schaffner T., Taylor K., Bartucci E. J., Fischer-Dzoga K., Beeson J. H., Glagov S., Wissler R. W. Arterial foam cells with distinctive immunomorphologic and histochemical features of macrophages. Am J Pathol. 1980 Jul;100(1):57–80. [PMC free article] [PubMed] [Google Scholar]
  24. Schonfeld G., Patsch W., Pfleger B., Witztum J. L., Weidman S. W. Lipolysis produces changes in the immunoreactivity and cell reactivity of very low density lipoproteins. J Clin Invest. 1979 Nov;64(5):1288–1297. doi: 10.1172/JCI109584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shechter I., Fogelman A. M., Haberland M. E., Seager J., Hokom M., Edwards P. A. The metabolism of native and malondialdehyde-altered low density lipoproteins by human monocyte-macrophages. J Lipid Res. 1981 Jan;22(1):63–71. [PubMed] [Google Scholar]
  26. White R., Lin H. S., Kuhn C., 3rd Elastase secretion by peritoneal exudative and alveolar macrophages. J Exp Med. 1977 Sep 1;146(3):802–808. doi: 10.1084/jem.146.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zilversmit D. B. Atherogenesis: a postprandial phenomenon. Circulation. 1979 Sep;60(3):473–485. doi: 10.1161/01.cir.60.3.473. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES