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. 1982 Aug;37(2):513–518. doi: 10.1128/iai.37.2.513-518.1982

Growth inhibition of oral streptococci in saliva by anionic proteins from two caries-free individuals.

R A Cowman, S S Baron, R J Fitzgerald, J L Danziger, J A Quintana
PMCID: PMC347564  PMID: 7118248

Abstract

Mixed saliva from two caries-free individuals possessed antimicrobial activity toward Streptococcus mutans and S. sanguis. This inhibitory activity was attributed to the presence of a group of four anionic proteins each of which strongly inhibited the growth of the oral streptococci in a saliva protein-based medium but not in a medium containing amino acids as a nitrogen source. These proteins, with isoelectric points of 4.70, 4.90, 4.98, and 5.05, respectively, neither reacted with antisera to immunoglobulin A, G, or M nor appeared to be functionally related to a number of salivary peroxidases, lactoferrin, or lysozyme. On this basis, they may represent a previously unreported group of growth-inhibitory antimicrobial factors occurring in the saliva of some individuals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnold R. R., Brewer M., Gauthier J. J. Bactericidal activity of human lactoferrin: sensitivity of a variety of microorganisms. Infect Immun. 1980 Jun;28(3):893–898. doi: 10.1128/iai.28.3.893-898.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnold R. R., Cole M. F., McGhee J. R. A bactericidal effect for human lactoferrin. Science. 1977 Jul 15;197(4300):263–265. doi: 10.1126/science.327545. [DOI] [PubMed] [Google Scholar]
  3. Arnold R. R., Mestecky J., McGhee J. R. Naturally occurring secretory immunoglobulin A antibodies to Streptococcus mutans in human colostrum and saliva. Infect Immun. 1976 Aug;14(2):355–362. doi: 10.1128/iai.14.2.355-362.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang J. Y. Amino-terminal analysis of polypeptide using dimethylaminoazobenzene isothiocyanate. Anal Biochem. 1980 Mar 1;102(2):384–392. doi: 10.1016/0003-2697(80)90172-4. [DOI] [PubMed] [Google Scholar]
  5. Cowman R. A., Baron S. J., Fitzgerald R. J. Utilization of hydroxyapatite adsorbable salivary proteins as growth substrates for plaque-forming oral streptococci. J Dent Res. 1981 Oct;60(10):1803–1808. doi: 10.1177/00220345810600101201. [DOI] [PubMed] [Google Scholar]
  6. Cowman R. A., Fitzgerald R. J., Perrella M. M., Cornell A. H. Human saliva as a nitrogen source for oral streptococci. Caries Res. 1977;11(1):1–8. doi: 10.1159/000260242. [DOI] [PubMed] [Google Scholar]
  7. Cowman R. A., Perrella M. M., Fitzgerald R. J. Influence of incubation atmosphere on growth and amino acid requirements of Streptococcus mutans. Appl Microbiol. 1974 Jan;27(1):86–92. doi: 10.1128/am.27.1.86-92.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cowman R. A., Schaefer S. J., Fitzgerald R. J., Rosner D., Shklair I. L., Walter R. G. Differential utilization of proteins in saliva from caries-active and caries-free subjects as growth substrates by plaque-forming streptococci. J Dent Res. 1979 Oct;58(10):2019–2027. doi: 10.1177/00220345790580101501. [DOI] [PubMed] [Google Scholar]
  9. Cowman R. A., Schaefer S. J., Fitzgerald R. J. Specificity of utilization of human salivary proteins for growth by oral streptococci. Caries Res. 1979;13(4):181–189. doi: 10.1159/000260399. [DOI] [PubMed] [Google Scholar]
  10. Cowman R. A., Speck M. L. Low temperature as an environmental stress on microbial enzymes. Cryobiology. 1969 Mar-Apr;5(5):291–299. doi: 10.1016/s0011-2240(69)80457-8. [DOI] [PubMed] [Google Scholar]
  11. Cowman R. A., Swaisgood H. E., Speck M. L. Proteinase enzyme system of lactic streptococci. II. Role of membrane proteinase in cellular function. J Bacteriol. 1967 Oct;94(4):942–948. doi: 10.1128/jb.94.4.942-948.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Iacono V. J., MacKay B. J., DiRienzo S., Pollock J. J. Selective antibacterial properties of lysozyme for oral microorganisms. Infect Immun. 1980 Aug;29(2):623–632. doi: 10.1128/iai.29.2.623-632.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mandel I. D. Dental caries. Am Sci. 1979 Nov-Dec;67(6):680–688. [PubMed] [Google Scholar]
  14. Michalek S. M., McGhee J. R., Mestecky J., Arnold R. R., Bozzo L. Ingestion of Streptococcus mutans induces secretory immunoglobulin A and caries immunity. Science. 1976 Jun 18;192(4245):1238–1240. doi: 10.1126/science.1273589. [DOI] [PubMed] [Google Scholar]
  15. Orstavik D., Kraus F. W., Henshaw L. C. In vitro attachment of streptococci to the tooth surface. Infect Immun. 1974 May;9(5):794–800. doi: 10.1128/iai.9.5.794-800.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pilz H., O'Brien J. S., Heipertz R. Human saliva peroxidase: microanalytical isoelectric fractionation and properties in normal persons and in cases with neuronal ceroid-lipofuscinosis. Clin Biochem. 1976 Apr;9(2):85–88. doi: 10.1016/s0009-9120(76)80023-9. [DOI] [PubMed] [Google Scholar]
  17. Pruitt K. M., Adamson M., Arnold R. Lactoperoxidase binding to streptococci. Infect Immun. 1979 Jul;25(1):304–309. doi: 10.1128/iai.25.1.304-309.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Socransky S. S., Manganiello A. D., Propas D., Oram V., van Houte J. Bacteriological studies of developing supragingival dental plaque. J Periodontal Res. 1977 Mar;12(2):90–106. doi: 10.1111/j.1600-0765.1977.tb00112.x. [DOI] [PubMed] [Google Scholar]
  19. Sorrells K. M., Cowman R. A., Swaisgood H. E. Hydrolysis of alpha(s, 1)-Casein B by Streptococcus lactis Membrane Proteinase. J Bacteriol. 1972 Oct;112(1):474–479. doi: 10.1128/jb.112.1.474-479.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tenovuo J. Different molecular forms of human salivary lactoperoxidase. Arch Oral Biol. 1981;26(12):1051–1055. doi: 10.1016/0003-9969(81)90116-3. [DOI] [PubMed] [Google Scholar]
  21. Tenovuo J., Knuuttila M. L. The antibacterial action of the various components of the lactoperoxidase system on a cariogenic strain of Streptococcus mutans. J Dent Res. 1977 Dec;56(12):1603–1607. doi: 10.1177/00220345770560123201. [DOI] [PubMed] [Google Scholar]

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